儿童肝母细胞瘤肝组织中GRP78和GRP94表达及其临床病理意义

Expression of GRP78 and GRP94 in the liver tissues and their clinicopathological significance in children with hepatoblastoma

摘要:

目的：研究肝母细胞瘤（HB）患儿肝组织中葡萄糖调节蛋白78和94 （GRP78和GRP94）表达水平及其临床病理意义。方法：15例儿童HB和10例正常肝组织标本常规制作石蜡包埋切片，用EnVsion免疫组织化学法，检测GRP78和GRP94表达。结果：HB患儿肝组织中GRP78和GRP94表达阳性率明显高于正常肝组织（53% vs 10%, 60% vs 10%； 均P<0.05）。无淋巴结转移及临床分期Ⅰ～Ⅱ期病例GRP78表达阳性率明显低于淋巴结转移及临床分期Ⅲ～Ⅳ期病例(P<0.05)。无淋巴结转移及临床分期Ⅰ～Ⅱ期病例GRP94表达阳性率低于淋巴结转移及临床分期Ⅲ～Ⅳ期病例, 但差异无统计学意义(P>0.05)。结论：GRP78和GRP94表达在儿童HB发生、进展方面可能有重要作用。［中国当代儿科杂志，2010，12（8）：634-636］

Abstract:

OBJECTIVE: To study the expression of glucose-regulated protin 78 (GRP78) and glucose-regulated protin 94 (GRP94) in the liver tissues from children with hepatoblastoma (HB) and to investigate the possible clinicopathological values of GRP78 and GRP94 in HB. METHODS: Liver tissue specimens from 15 children with HB and 10 specimens of normal liver tissues were obtained. EnVison immunohistochemistry was used to detect the expression of GRP78 and GRP94 in the conventional paraffin-embedded liver sections. RESULTS: The positive rates of GRP78 expression (53% vs 10%; P<0.05) and GRP94 expression (60% vs 10%; P<0.05) in HB liver tissues were significantly higher than those in the normal liver tissues. The positive rates of GRP78 expression in the cases without lymphnode metastasis or in clinical stage I-II were significantly lower than those in the cases with lymphnode metastasis or in clinical stage III-IV (P<0.05). GRP94 showed a decreased tendency of positive expression in the cases without lymphnode metastasis or in clinical stage I-II when compared with the cases with lymphnode metastasis or in clinical stage III-IV, although there were no statistical differences between them. CONCLUSIONS: GRP78 and GRP94 expression might play important roles in the pathogenesis and progression of pediatric HB.［Chin J Contemp Pediatr, 2010, 12 (8):634-636］