母子隔离应激对幼鼠癫癎易感性的影响

Effect of maternal isolation stress on epilepsy susceptibility in young rats

摘要:

目的：初步探讨母子隔离应激对幼鼠癫癎易感性影响及其潜在机制。方法：60只Sprague-Dawley幼鼠随机分成3组：对照组、生后第2~17天每天母子隔离15 min组和隔离3 h组。在生后18 d分别行杏仁核刺激诱导惊厥实验，灌注取脑，免疫组化检测海马GABAAα1亚单位表达情况。结果：与对照组比较，每天母子隔离3 h组幼鼠的体重减轻， 杏仁核点燃惊厥阈值降低，完全点燃所需刺激次数减少，惊厥发作程度加重，海马CA1区GABAAα1亚单位表达减少（P<0.05），而每天母子隔离15 min组与对照组幼鼠在以上几个方面比较则差异无统计学意义（P>0.05）。结论：早期每天母子隔离3 h的应激对幼鼠脑发育、癫癎易感性产生不良影响，海马GABAAα1亚单位表达减少可能作为一个重要的潜在机制。［中国当代儿科杂志，2010，12（8）：662-665］

Abstract:

OBJECTIVE: To study the effect of maternal isolation stress on the epilepsy susceptibility in young rats and the possible mechanism. METHODS: Sixty Sprague-Dawley young rats were randomly divided into a normal control and two maternal isolation groups that were subjected to maternal isolation for 15 min or 3 hrs daily on postnatal days 2-17. On postnatal day 18, an amygdala kindling test was performed to induce seizures. The expression of GABAA receptor α1 in the hippocampus was determined by immunohistochemisty. RESULTS: The weights were reduced, the threshold of amygdala kindling and the stimulation number for full kindling decreased significantly, and seizures were more severe in the maternal isolation 3 hrs group compared with the normal control group. The expression of GABAA receptor α1 in the hippocampus CA1 area in the maternal isolation 3 hrs group decreased significantly compared with that in the normal groups. There were no significant differences in the aspects above mentioned between the maternal isolation 15 min and normal control groups. CONCLUSIONS: The stress of early daily maternal isolation for 3 hrs may affect adversely brain development and increase epilepsy susceptibility in young rats. The decreased expression of GABAA receptor α1 in the hippocampus may contribute to the potential mechanism.［Chin J Contemp Pediatr, 2010, 12 (8):662-665］