谷氨酰胺对内毒素血症幼鼠肠屏障的保护作用

Effect of oral glutamine on intestinal barrier function in yaung rats with endotoxemia

摘要:

目的：观察内毒素血症幼鼠经谷氨酰胺（Gln）干预后血浆D-乳酸、血浆及小肠二胺氧化酶（DAO）浓度变化，以探讨Gln对肠屏障功能的干预作用。方法：18日龄Wistar大鼠80只，随机分为内毒素血症组、Gln干预组，每组40只，各组根据注射内毒素后取标本时间分为1.5 h、 6 h、 24 h、 72 h和7 d等5个亚组（n=8）。Gln干预组在注射内毒素后立即予以Gln口服（2 g/kg）,随后每天1次。各组分别取血浆及小肠匀浆，测定血浆DAO活性及D乳酸、小肠匀浆DAO值。结果：① Gln干预组6 h、 72 h血浆DAO活性明显低于内毒素组（P<0.05）。② Gln干预组 6 h、 24 h、 72 h及7 d的肠组织DAO活性较内毒素组均明显增高（P<0.05，P<0.01）。③ Gln干预组6 h、 24 h、 72 h、 7 d血浆D-乳酸含量明显低于内毒素组（P<0.01）。结论：Gln能降低内毒素血症大鼠肠道黏膜通透性，从而具有保护肠屏障功能。［中国当代儿科杂志，2010，12（10）：809-811］

Abstract:

OBJECTIVE: To study the effect of glutamine on intestinal barrier function by examining the changes of plasma D-lactic levels and diamine oxidase (DAO) levels in plasma and intestinal tissue after glutamine intervention in young rats with endotoxemia. METHODS: Eighty 18-day-old rats were randomly divided into endotoxemia and glutamine intervention groups (n=40 each). Endotoxemia was induced by lipopolysaccharide (LPS) injection. Plasma and small intestine homogenate were collected 1.5, 6, 24 and 72 hrs and 7 days after LPS injection. The glutamine intervention group was immediately administered with oral glutamine (2 g/kg) after LPS injection. Afterwards, glutamine was administered once daily. Plasma D-lactic and DAO levels and intestinal DAO levels were measured. RESULTS: Plasma DAO activity in the glutamine intervention group was significantly lower than that in the endotoxemia group 6 and 72 hrs after LPS injection (P<0.05). In contrast, the intestinal DAO activity in the glutamine intervention group was significantly higher than that in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.05 or 0.01). Plasma D-lactic levels in the glutamine intervention group were significantly lower than those in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.01). CONCLUSIONS: Glutamine may reduce the permeability of intestinal mucosa, and thus provides protective effects on intestinal barrier function in rats with endotoxemia.［Chin J Contemp Pediatr, 2010, 12 (10):809-811］