不同增生程度星形胶质细胞对癫癎大鼠多药耐药基因MDR表达的影响

Effect of astrocytes with different degrees of proliferation on multidrug resistance gene expression in rats with epilepsy

摘要:

目的：探讨星形胶质细胞增生与癫癎大鼠多药耐药基因（MDR）表达的关系。方法：将新生Sprague-Dawley大鼠大脑皮层星形胶质细胞分离、纯化并培养至第3代后，采用肿瘤坏死因子（TNF-α）分别作用2 h、24 h 及48 h以刺激星形胶质细胞增生。将不同增生程度的星形胶质细胞培养液分别注入到癫癎大鼠侧脑室，用PCR法、免疫组化法和免疫印记法测定脑组织MDR产物P-糖蛋白（P-gp）表达。结果：TNF-α刺激后2 h胶质细胞开始增生，24 h达高峰；增生的胶质细胞培养液可使癫癎大鼠MDR表达上调，表达量与增生程度一致。结论：星形胶质细胞增生可使癫癎大鼠MDR基因表达上调，可能参与了难治性癫癎的形成。［中国当代儿科杂志，2010，12(11)：908-911］

Abstract:

OBJECTIVE: To study the relationship of activated astrocytes and multidrug resistance gene (MDR) expression in rats with epilepsy. METHODS: Astrocytes of neonatal Sprague-Dawley rats were separated and cultured. The cultured cells of passage 3 were activated by TNF-α for 2, 24 or 48 hrs. The culture media of cells with different degrees of proliferation were infused to the lateral cerebral ventricle of rats with epilepsy. The expression of MDR in the brain tissue was ascertained by PCR, immunocytochemistry and Western blot. RESULTS: After 2 hrs of TNF-α stimulation, astrocytes began to proliferate, and reached a peak at 24 hrs. The expression of MDR in the brain tissue increased after infusion of culture medium of proliferated astrocytes in the TNF stimulation group compared with that in the control group without TNF stimulation. The level of MDR expression in the TNF stimulation group was positively correlated with the degrees of cell proliferation. CONCLUSIONS: Proliferation of astrocytes can increase the expression of MDR in rats with epilepsy and is probably involved in the development of refractory epilepsy.［Chin J Contemp Pediatr, 2010, 12 (11):908-911］