T系和B系急性淋巴细胞白血病ID4甲基化状态分析

ID4 methylation patterns in childhood T line and B line lymphocytic leukemia

摘要:

目的探讨DNA结合抑制因子4（ID4）基因甲基化与T系、B系及T/B双表达儿童急性淋巴细胞白血病（ALL）的关系。方法采用甲基化特异性聚合酶链式反应（MS-PCR）对18例初发ALL患儿进行ID4基因启动子区甲基化状况分析。18例ALL患儿中T系（T-ALL）2例，B系（B-ALL）13例，T/B双表达（T/B-ALL）3例。以34 例同期住院的非肿瘤性疾病患儿为对照组。结果ID4基因启动子区在初发ALL患儿中的完全甲基化率（15/18，83%）显著高于部分甲基化率（3/18，17％），P＜0.05。T-ALL、B-ALL和T/B-ALL患儿的完全甲基化率分别为50%,85%，100%，显著高于对照组（18%；P<0.05），部分甲基化率和非甲基化率低于对照组（P<0.05）。T-ALL、B-ALL及T/B-ALL 患儿之间ID4启动子区甲基化状态差异无统计学意义（P>0.05）。结论 ID4基因的甲基化可能与儿童ALL发病有关，T-ALL、B-ALL及T/B-ALL之间的ID4甲基化状态一致。［中国当代儿科杂志，2010，12(12)：940-942］

Abstract:

OBJECTIVE: To study the relationship of methylation of inhibitor of DNA binding 4 (ID4) gene core promoter region with childhood T line, B line and T/B acute lymphocytic leukemia (ALL). METHODS: Methylation-specific polymerase chain reaction (MS-PCR) was used to detect the methylation status of ID4 promoter region in 18 children with newly-diagnosed ALL (2 cases of T-ALL, 13 cases of B-ALL and 3 cases of T/B-ALL). Thirty-four hospitalized children with non-tumor disease served as the control group. RESULTS: The complete methylation rate of ID4 gene promoter region (15/18, 83%) was significantly higher than the partial methylation rate (3/18, 17%) in the 18 ALL children (P<0.05). The complete methylation rate of ID4 gene promoter region in children with T-ALL, B-ALL and T/B-ALL (50%, 85% and 100% respectively) was significantly higher than that in the control group (18%; P<0.05). In contrast, the partial methylation rate and non-methylation rate in the three ALL groups were significantly lower than those in the control group (P<0.05). There were no statistically significant differences in the methylation patterns among the B-ALL, T-ALL and T/B-ALL cases. CONCLUSIONS: The methylation of ID4 promoter region may be related to the pathogenesis of childhood ALL. The methylation patterns of ID4 promoter region are identical in BALL, TALL and T/BALL.［Chin J Contemp Pediatr, 2010, 12 (12):940-942］