三种方法建立神经元与星形胶质细胞共培养模型的对比研究

A comparative study on three models of co-culture of neurons and astrocytes

摘要:

目的：比较神经元和星形胶质细胞共培养的3种不同方法，旨在获得高纯度的神经元。方法：采用新生大鼠作为神经元和星形胶质细胞体外培养的细胞来源，用混合培养、Banker共培养方法和插入式培养皿的方法建立神经元和星形胶质细胞共培养模型。结果：混合培养的神经元和星形胶质细胞同时生长在一个盖玻片上，不易控制胶质细胞的生长速度，神经元纯度不高；Banker共培养方法和插入式培养皿的方法均可获得高纯度的神经元，但 Banker 程序复杂；插入式培养皿的方法简化了 Banker 共培养程序。结论：利用插入式培养皿建立神经元和星形胶质细胞共培养体系，是一种值得推广的可用于体外细胞共培养相关研究工作的有效方法。［中国当代儿科杂志，2010，12(12)：984-987］

Abstract:

OBJECTIVE: To find a better method for harvesting highly purified neurons by comparing three methods used for co-culture of neurons and astrocytes. METHODS: The co-culture models of neurons and astrocytes were established by primary culture, Banker's co-culture method or Transwell cell-culture inserts. The neurons and astrocytes cultured in vitro were from neonatal rats. RESULTS: The highly purified neurons were not harvested by primary culture because the neurons and astrocytes grew on the same cover slip and it was difficult to control the growth velocity of astrocytes. The highly purified neurons were harvested by Banker's co-culture method or the method using Transwell cell-culture inserts, but the procedure of the former was more complicated than that of the later. CONCLUSIONS: The culture method using Transwell cell-culture inserts is recommended for the establishment of the co-culture system of neurons and astrocytes.［Chin J Contemp Pediatr, 2010, 12 (12):984-987］