TGF-β1影响肺成纤维细胞结缔组织生长因子基因表达的研究

Effects of TGF-β1 on gene expression of connective tissue growth factor in lung fibroblasts

摘要:

目的：研究转化生长因子-β1（TGF-β1）对体外培养早产鼠的肺成纤维细胞（LFs）结缔组织生长因子（CTGF）基因表达的影响。方法：以孕 19 d Wistar大鼠剖宫取出的仔鼠为对象，原代培养LFs，实验组分别采用不同浓度（1 ng/mL、5 ng/mL、10 ng/mL）和不同作用时间（12 h、24 h、48 h）TGF-β1刺激LFs，对照组无血清培养基培养（即0 ng/mL）。应用 RT-PCR 方法检测 LFs 的 CTGF mRNA 表达。结果：与对照组比较，实验组LFs的CTGF mRNA水平增高(P<0.05)，且随TGF-β1作用浓度的增加、作用时间的延长CTGF mRNA表达逐渐上升，差异有统计学意义（P<0.05）。结论：TGF-β1 能刺激肺成纤维细胞中CTGF基因表达，并且以剂量和时间依赖方式上调 CTGF 基因表达。

Abstract:

OBJECTIVE: To study the effects of transforming growth factor-β1 (TGF-β1) on the gene expression of connective tissue growth factor (CTGF) in cultured lung fibroblasts of embryonic rats in vitro. METHODS: Wistar rats of embryonic 19 days were used for primary culture of lung fibroblasts (LFs). The cells in the experimental group were treated by different concentrations (1, 5 or 10 ng/mL) and different durations (12, 24 or 48 hrs) of TGF-β1 to stimulate the LFs. The cells in the control group were cultured in serum-free medium. RT-PCR method was applied to detect CTGF mRNA expression in LFs. RESULTS: Compared with the control group, the levels of CTGF mRNA in LFs in the experimental group increased significantly (P<0.05). CTGF mRNA expression gradually increased with increasing concentration and duration of TGF-β1 treatment (P<0.05). CONCLUSIONS: TGF-β1 can stimulate CTGF gene expression in LFs and increase CTGF gene expression in a dose-and time-dependent manner.