r-HuEPO对癫癎持续状态大鼠血清NSE S-100β蛋白和MBP表达的影响

Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100β protein and myelin basic protein in rats following status epilepticus

摘要:

目的：通过观察重组人促红细胞生成素(r-HuEPO)对幼年大鼠癫癎持续状态（SE）后24 h血清神经元特异性烯醇化酶(NSE)、S-100β蛋白和髓鞘碱性蛋白(MBP)表达的影响，以观察其在癫癎性脑损伤中的可能作用。方法：40只19～21日龄Sprague-Dawley雄性大鼠随机分为正常对照组(CON组)、SE组、r-HuEPO预处理+SE组(SE-EPO组)、r-HuEPO组(EPO组)（n=10），采用氯化锂匹鲁卡品点燃制成SE模型。SE-EPO组和EPO组在制模前4 h腹腔注射r-HuEPO 500 IU/kg。在SE后24 h测定血清中NSE、S-100β蛋白、MBP含量的变化。结果：与CON、EPO组相比，SE组血清NSE、S-100β蛋白、MBP浓度均显著升高（P＜0.05）；与SE组比较，SE-EPO组NSE、S-100β蛋白、MBP均显著下降（P＜0.05）。结论：r-HuEPO可降低癫癎后神经组织损伤指标表达，对癫癎性脑损伤可能具有早期保护作用。

Abstract:

OBJECTIVE: This study examined the effect of recombinant human erythropoietin (r-HuEPO) on the serum levels of neuron-specific enolase (NSE), S-100β protein and myelin basic protein (MBP) in young rats 24 hrs after lithium-pilocarpine-induced status epilepticus (SE) in order to study the potential role of r-HuEPO in epileptic brain damage. METHODS: Forty 19-21-day-old male Sprague-Dawley (SD) rats were randomly divided into four groups (n=10): normal control group, SE, r-HuEPO pretreated-SE and r-HuEPO. SE was induced by lithium-pilocarpine. R-HuEPO (500 IU/kg) was intraperitoneally injected in the r-HuEPO pretreated-SE and r-HuEPO groups 4 hrs before SE. Serum levels of NSE, S-100β and MBP were determined 24 hrs after the SE event. RESULTS: Serum levels of NSE, S-100β and MBP in the SE group increased significantly compared with those in the normal control and the r-HuEPO groups (P＜0.05). The r-HuEPO pretreated-SE group showed significantly decreased serum levels of NSE, S-100β and MBP compared with the SE group (P＜0.05). CONCLUSIONS: r-HuEPO may reduce the expression of NSE, S-100β and MBP and thus might provide an early protective effect against epileptic brain injury.