Clara细胞分泌蛋白10在5岁以下喘息儿童外周血中的表达

Expression of serum Clara cell secretory protein 10 in wheezing children under 5 years old

摘要:

目的：探讨外周血中Clara细胞分泌蛋白10（CC10）和血清总IgE浓度在5岁以下喘息儿童中的表达。方法：随机选取5岁以下反复喘息患儿59例，分为有特应质高危因素的喘息Ⅰ组（n=33）和无特应质高危因素的喘息Ⅱ组（n=26），对照组为近期无感染疾病史的外科术前患儿(n=23)。采用固相夹心酶免疫吸附实验（ELISA）测定3组患儿外周血CC10与IgE水平。结果：喘息Ⅰ组、Ⅱ组CC10水平(3.95±1.26, 5.41±1.64 ng/mL)均低于对照组(8.72±2.23 ng/mL),差异有统计学意义(P<0.01)；喘息Ⅰ组CC10水平低于喘息Ⅱ组(P＜0.05)。喘息Ⅰ组IgE水平高于喘息Ⅱ组和对照组,差异有统计学意义（P＜0.05）,喘息Ⅱ组和对照组之间差异无统计学意义（P＞0.05）。喘息Ⅰ组血清CC10与IgE呈负相关（r=-0.912, P＜0.01）。结论：外周血CC10水平在5岁以下患儿喘息发作期显著降低,有特应质高危因素的患儿降低更为明显，并与外周血IgE水平呈负相关。

Abstract:

OBJECTIVE: To study the expression of serum Clara cell secretory protein 10 (CC10) and total IgE concentration in wheezing children under 5 years old. METHODS: Fifty-nine children with recurrent wheezing under 5 years old were classified into two groups: wheezing group 1 with atopic high risks (n=33) and wheezing group 2 without atopic high risks (n=26). Twenty-three children without infectious diseases served as a control group. Serum levels of CC10 and IgE were measured using a solid-phase sandwich ELISA. RESULTS: The serum levels of CC10 in wheezing group 1 (3.95±1.26 ng/mL) and wheezing group 2 (5.41±1.64 ng/mL) were significantly lower than those in the control group (8.72±2.23 ng/mL; P<0.01). The wheezing group 1 showed more decreased serum levels of CC10 compared with wheezing group 2 (P<0.05). The serum IgE levels in wheezing group 1 were significantly higher than those in wheezing group 2 and the control group (P<0.05). There were no significant differences in serum IgE levels between the wheezing group 2 and control group. There was a negative correlation between serum levels of CC10 and IgE in wheezing group 1 (r=-0.912, P﹤0.01). CONCLUSIONS: Serum CC10 levels decrease remarkably in wheezing children, and more significant decrease is noted in patients with atopic high risks. Serum CC10 levels are negatively correlated to serum IgE levels in patients with atopic high risks.