可溶性DLL1在儿童颅内感染性疾病诊断中的意义

Significance of soluble DLL1 in diagnosis of intracranial infectious diseases in children

摘要:

目的：探讨脑脊液（CSF）及血清中可溶性DLL1的水平在儿童颅内感染诊断中的意义。方法：选择颅内感染患儿50例（结脑20例，病脑20例，化脑10例）以及非颅内感染患儿（对照组）20例，采用酶联免疫吸附试验定量测定患儿CSF及血清中可溶性DLL1的水平。结果：结脑组患儿CSF中可溶性DLL1的水平明显高于病脑组、化脑组及对照组（2.89±1.72 ng/mL vs 0.14±0.14 ng/mL, 0.27±0.21 ng/mL, 0.13±0.12 ng/mL; P＜0.01）；结脑组患儿血清中可溶性DLL1的水平也明显高于病脑组、化脑组及对照组（12.61±6.45 ng/mL vs 2.28±2.27 ng/mL, 2.38±1.79 ng/mL, 2.26±2.10 ng/mL; P＜0.01）；病脑组、化脑组与对照组相比，CSF及血清中可溶性DLL1的水平差异无统计学意义（P>0.05）。结论：可溶性DLL1的检测作为一种新的指标，在结脑诊断中可能有潜在重要价值。

Abstract:

OBJECTIVE: To investigate the significance of soluble DLL1 (Delta-like-1) levels of cerebrospinal fluid (CSF) and serum in the diagnosis of intracranial infection in children. METHODS: Fifty children with intracranial infection, including 20 cases of tuberculous meningitis (TM), 20 cases of viral meningitis (VM) and 10 cases of purulent meningitis (PM), and 20 children without intracranial infection (control group) were enrolled. The levels of soluble DLL1 in CSF and serum were measured using ELISA. RESULTS: The level of CSF soluble DLL1 in the TM group was significantly higher than that in the VM, PM and control groups (2.89±1.72 ng/mL vs 0.14±0.14 ng/mL, 0.27±0.21 ng/mL, 0.13±0.12 ng/mL; P<0.01). The level of serum soluble DLL1 in the TM group was also significantly higher than that in the VM, PM and control groups (12.61±6.45 ng/mL vs 2.28±2.27 ng/mL, 2.38±1.79 ng/mL, 2.26±2.10 ng/mL; P<0.01). The levels of soluble DLL1 in the CSF and serum in the VM and PM groups were not significantly different from those in the control group. CONCLUSIONS: Soluble DLL1 as a novel indicator might have potentially important value in the diagnosis of TM.