过敏性紫癜P-选择素基因 -2123位点多态性检测与临床分析

P-selectin gene -2123 polymorphism in children with Henoch-Schonlein purpura

摘要:

目的：研究P-选择素基因 -2123位点多态性与儿童过敏性紫癜（HSP）发病的相关性。方法应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)分析方法对86例HSP患儿（其中40例合并肾炎）和70例健康对照进行基因多态性检测。结果与对照组比较，HSP患儿P-选择素基因 -2123位点GG基因型频率和G等位基因频率均明显增高（P＜0.05）。合并肾炎患儿与未合并肾炎患儿比较，该位点各基因型频率及等位基因频率差异均无统计学意义（P＞0.05）。结论 P-选择素基因 -2123位点多态性可能与儿童HSP发病有关。

Abstract:

OBJECTIVE: To investigate whether P-selectin gene -2123 polymorphism is associated with the pathogenesis of Henoch-Schonlein purpura (HSP) in children. METHODS: Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) is used to identify the distribution of allele and genotype frequencies of P-selectin gene promoter -2123 polymorphism in 86 children with HSP (including 40 cases of purpura nephritis) and 70 healthy controls. RESULTS: Compared with the healthy controls, the frequencies of GG genotype and G allele of P-selectin promoter -2123 in children with HSP increased significantly (P<0.05). There were no significant differences in P-selectin promoter -2123 genotype and allele frequencies between the patients with and without nephritis. CONCLUSIONS: P-selectin gene promoter -2123 polymorphism appears to be associated with the pathogenesis of HSP in children.