特发性血小板减少性紫癜儿童外周血调节性T细胞的变化

Levels of regulatory T cells in peripheral blood of children with idiopathic thrombocytopenic purpura

摘要:

目的：研究特发性血小板减少性紫癜(ITP)患儿外周血CD4+CD25+CD127-及CD3+CD4-CD8-调节性T细胞（Treg）的变化及意义。方法：采用免疫荧光流式细胞技术检测33例ITP患儿及21例正常儿童外周血CD4+CD25+CD127-及CD3+CD4-CD8- Treg水平。结果：ITP患儿CD4+CD25+CD127-及CD3+CD4-CD8- Treg百分比明显低于正常儿童，分别为(2.7±1.7)％ vs (4.8±1.6)％；(5.2±3.1)％vs (8.1±3.5)％(P<0.01)。结论：ITP患儿CD4+CD25+CD127-及CD3+CD4-CD8- Treg百分率降低，提示其可能参与了ITP的发病机制。

Abstract:

OBJECTIVE: To study the levels of CD4+CD25+CD127- and CD3+CD4-CD8- regulatory T (Treg) cells in peripheral blood of children with idiopathic thrombocytopenic purpura (ITP). METHODS: The flow cytometry was used to detect the expression of CD4+CD25+CD127- and CD3+CD4-CD8- Treg cells in peripheral blood of 33 children with ITP and 21 healthy children. RESULTS: The expression levels of CD4+CD25+CD127-［(2.7±1.7)% vs (4.8±1.6)%; P<0.01］ and CD3+CD4-CD8-［(5.2±3.1)% vs (8.1±3.5)%; P<0.01］Treg cells in children with ITP were significantly lower than in the controls. CONCLUSIONS: The expression levels of CD4+CD25+CD127- and CD3+CD4-CD8- Treg cells decrease in children with ITP, suggesting that CD4+CD25+CD127- and CD3+CD4-CD8- Treg cells might play a role in the pathogenesis of ITP.