肿瘤坏死因子-α干预对热性惊厥患儿外周血单个核细胞ICAM-1和LFA-1表达的影响

Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures

摘要:

目的：探讨肿瘤坏死因子-α(TNF-α) 对热性惊厥(FS) 患儿外周血单个核细胞（PBMC）细胞间黏附分子-1(ICAM-1) 及配体淋巴细胞功能相关抗原-1(LFA-1) 表达的影响。方法：16例FS患儿随机分为FS对照组和TNF-α干预组。TNF-α干预组采用1.0 ng/mL 的TNF-α进行干预。对照组16例，为年龄和性别相匹配的同期体检健康儿童。进行外周血PBMC培养。流式细胞仪检测PBMC表面ICAM-1 和 LFA-1的表达。结果：FS 对照组在体外培养的PBMC表面ICAM-1和LFA-1表达水平分别为(20±9)% 和(43±16)%，明显高于正常对照组［(14±7)%，(30±16)%］（P＜0.05）；TNF-α干预组的PBMC表面ICAM-1表达水平［(27±11)%］明显高于FS对照组(P＜0.05)，LFA-1表达［（52±21）%］较FS对照组有增高趋势，但差异无统计学意义。结论：炎症介质TNF-α刺激可使FS患儿的LFA-1 和 ICAM-1表达上调。

Abstract:

OBJECTIVE: To study the effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells (PBMC) of children with febrile seizures (FS). METHODS: Sixteen children with FS and 16 age- and gender-matched healthy children were enrolled. The samples of PBMC from FS children were randomized into two groups with or without TNF-α treatment (TNF-α concentration 1.0 ng/mL). PBMC were purified and cultured with a conventional method in vitro. The expression of ICAM-1 and LFA-1 in PBMC was determined by flow cytometry (FCM). RESULTS: ICAM-1［(20±9)% vs (14±7)%)］and LFA-1［(43±16)% vs (30±16)%］expression in PBMC in the untreated FS group was significantly higher than that in the normal control group (P<0.05). Compared with the untreated FS group, the treatment with TNF-α remarkably increased the ICAM-1 expression［(27±11)%］(P<0.05). PBMC LFA-1 expression［(52±21)%］in the TNF-α-treated group was higher than that in the untreated FS group, although there were no statistical differences between the two groups. CONCLUSIONS: TNF-α treatment may increase LFA-1 and ICAM-1 expression in PBMC of children with FS.