血清血管内皮生长因子/内皮抑素比值与儿童急性白血病的相关性

Correlation between the ratio of serum vascular endothelial growth factor/endostatin and childhood acute leukemia

摘要:

目的：检测急性白血病（AL）患儿血清血管内皮生长因子（VEGF）、内皮抑素（ES）水平，探讨VEGF /ES (V/E) 比值与儿童AL的临床相关性。方法：采用酶联免疫双抗体夹心法检测35例AL患儿化疗前后血清VEGF、ES水平及V/E比值，并与30例健康儿童进行比较。结果：化疗前AL患儿血清中VEGF和ES水平分别为196±66 pg/mL和 31±7 ng/mL，显著高于对照组（29±10 pg/mL、19±4 ng/mL; P0.05）。35例AL患儿化疗前血清VEGF、V/E 比值与骨髓白血病细胞数呈正相关 (r=0.301和0.411，P<0.05)。结论：血清VEGF和ES与儿童AL的发生发展有关，V/E比值能更准确反映AL患儿疾病动态变化情况，有利于指导相关治疗。

Abstract:

OBJECTIVE: To investigate the correlation between the ratio of serum vascular endothelial growth factor (VEGF)/endostatin (ES) and childhood acute leukemia(AL). METHODS: Serum levels of VEGF and ES were measured using ELISA in 35 children with acute AL before and after chemotherapy. The ratio of VEGF/ES was calculated. Thirty healthy children served as the control group. RESULTS: The serum levels of VEGF (196±66 pg/mL vs 29±10 pg/mL) and ES (35±7 ng/mL vs 19±4 ng/mL) in the AL group before chemotherapy were significantly higher than those in the control group (P<0.01). The ratio of VEGF/ES in the AL group before chemotherapy was significantly higher than that in the control group (6.7±3.0 vs 1.6±0.7; P<0.01). In 20 children with AL who achieved complete remission, the serum levels of VEGF and ES and the VEGF/ES ratio were reduced after chemotherapy (83±35 pg/mL, 27±5 ng/mL, 3.1±1.3, respectively; P<0.01), although the serum levels of VEGF and ES were still higher than those in the control group (P<0.01). The VEGF/ES ratio in CR patients was not significantly different from that in the control group. The serum levels of VEGF (r=0.301, P=0.045) and the VEGF/ES ratio (r=0.411, P=0.015) were positively correlated with the count of blast cells in juvenile bone marrow in 35 children with AL before chemotherapy. CONCLUSIONS: Serum VEGF and ES levels are associated with the development of childhood AL. The VEGF/ES ratio can be used to evaluate the disease progression in children with AL.