脂联素基因多态性变化与儿童单纯性肥胖的关系

Association of adiponectin gene polymorphism with obesity in children

摘要:

目的：分析长沙市区儿童脂联素基因+45 位点单核苷酸多态性（SNP）基因型的分布频率，探讨其与儿童肥胖症的相关性。方法：随机抽取147例肥胖儿童及 118 例健康儿童，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测脂联素基因 SNP+45 多态性位点、双抗体夹心 ELISA 测定血清脂联素。同时进行腰围（WC）、腰臀比（WHR）、体脂百分比（%BF）、收缩压（SBP）、舒张压（DBP）、空腹血糖（FPG）、血清甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、空腹胰岛素水平（FINS）、稳态模型胰岛素抵抗指数（HOMA-IR）的测定。结果：肥胖组和正常组的SNP + 45 多态性发生频率分别为 40.5% 和 25.4%，两组间基因型分布与等位基因频率差异均有统计学意义（P<0.05）。肥胖儿童中，TT 基因型与 TG、GG 型相比，其脂联素水平高，而 %BF、HOMA-IR、TC 和 LDL-C 水平低，差异有统计学意义（P<0.05）。结论：脂联素基因SNP+45 变异可能与儿童单纯性肥胖相关，T→G 突变可增加儿童肥胖症的发生，同时使脂联素水平下降。

Abstract:

OBJECTIVE: To study the distribution characteristics of adiponectin gene +45 single nucleotide polymorphisms (SNP) in Chinese children, and to determine the role of adiponectin gene +45 polymorphisms in the pathogenesis of childhood obesity. METHODS: A total of 147 Chinese obese and 118 healthy children were randomly selected and enrolled to identify adiponectin gene SNP+45 polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Plasma adiponectin levels were determined using ELISA. Waist circumference (WC), waist to hip ratio (WHR), percentage of body fat (%BF), systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting plasma glucose (FPG), serum triglycerides (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), plasma fasting insulin (FINS), and homeostasis model assessment of insulin resistance (HOMA-IR) were measured. RESULTS: The allelic frequency of adiponectin gene SNP+45 in children with obesity and healthy controls were 40.5% and 25.4%, respectively. There were significant differences in the distribution of genotypes and the allelic frequency between the two groups (P<0.05). The plasma adiponectin levels were significantly higher, in contrast, %BF, HOMA-IR, TC and LDL-C levels were significantly lower in obese children with TT genotype than those in obese children with TG or GG genotype. CONCLUSIONS: The adiponectin gene SNP+45 polymorphism may be associated with pathogenesis of obesity in children. T→G variance may be associated an increased risk of childhood obesity and result in a decreased level of adiponectin.