黄芩苷对自身免疫性脑脊髓炎大鼠细胞凋亡的影响

Effects of baicalin on apoptosis in rats with autoimmune encephalomyelitis

摘要:

目的：观察黄芩苷（BAC）对自身免疫性脑脊髓炎（EAE）大鼠的疗效及其对脊髓炎症细胞凋亡的影响。方法：44 只Wistar大鼠随机分为正常对照组（NC, n=10）、EAE 组(n=12)、地塞米松治疗组（DXM, n=12）、BAC 治疗组(n=10)。 DXM 组和 BAC 组在抗原免疫后1周分别予以DXM (1 mg/kg) 和BAC (200 mg/kg) 治疗7 d；观察各组动物脊髓病理变化及髓鞘碱性蛋白（MBP）表达的情况和脊髓炎症细胞凋亡情况。结果：EAE 组、DXM 组和 BAC 组较NC组体重均下降，BAC 组体重较 EAE 组及DXM组明显上升（P<0.05）。DXM 组和 BAC 组大鼠神经功能评分明显优于EAE组（P<0.05）；DXM 组和 BAC 组脊髓 MBP 阳性数均较 EAE 组显著增多（P<0.05）。DXM 组和 BAC 组脊髓炎症细胞凋亡数均较EAE 组显著增多（P<0.05）。 结论：BAC对EAE大鼠有治疗作用，并且能促进大鼠脊髓炎症细胞凋亡。

Abstract:

OBJECTIVE: To study the therapeutic efficacy of baicalin and its effect on apoptosis of inflammatory cells in spinal cords in Wistar rats with autoimmune encephalomyelitis (EAE). METHODS: Forty-four rats were randomly divided into four groups: normal control group (control, n=10), EAE group (n=12), and two intervention groups with dexamethasone (DXM) or baicalin. Seven days after immunization, the two intervention groups were injected intraperitoneally with DXM (1 mg/kg) and baicalin (200 mg/kg) for 1 week, respectively. The spinal cords were removed 14 days after immunization, and stained with hematoxylin and eosin. MBP expression in spinal cords was detected by immunohistochemistry. The apoptosis of inflammatory cells in spinal cords was detected by TUNEL. RESULTS: The weight gain rate in the untreated EAE and the DXM or baicalin intervention groups were significantly lower than that in the control group (P<0.05). The weight gain rate in the baicalin intervention group was significantly higher than that in the untreated EAE and the DXM intervention groups (P<0.05). The scores of neurological function in the two intervention groups were significantly higher than that in the untreated EAE group (P<0.05). DXM or baicalin treatment significantly increased the MBP expression compared with the untreated EAE group (P<0.05). The apoptosis of inflammatory cells increased more in the DXM and the baicalin intervention groups compared with the untreated EAE groups (P<0.05). CONCLUSIONS: Baicalin has protective effects against EAE in rats. It can promote the apoptosis of inflammatory cells in spinal cords.