C-kit和Cx43在新生儿自发性胃穿孔中的表达

Expression of c-kit and Cx43 in neonates with spontaneous gastric perforation

摘要:

目的：分析 c-kit和缝隙连接蛋白43（Cx43）在新生儿自发性胃穿孔（spontaneous neonatal gastric perforation, SNGP）中的表达情况，探讨Cajal间质细胞（interstitial cell of Cajal, ICC）在新生儿SNGP中的意义。方法：收集2001~2010年手术治疗的19例SNGP胃组织标本，以8例无消化道畸形新生儿尸检胃组织作为对照，采用免疫组织化学SP法进行c-kit和Cx43检测。结果：SNGP患儿胃壁穿孔处肌层缺失，穿孔周围肌束明显变薄。与对照组比较，SNGP患儿胃组织中c-kit阳性细胞以及Cx43阳性细胞表达明显减少（P＜0.01）。结论：新生儿SNGP与胃壁ICC数量减少、缝隙连接结构破坏有关。

Abstract:

OBJECTIVE: To study the clinical significance of interstitial cell of Cajal (ICC) in spontaneous neonatal gastric perforation by examining the expression of c-kit and Cx43 in neonates with this disorder. METHODS: The gastric specimens of 19 cases of neonatal gastric perforation from 2001 to 2010 and 8 cases of accidental death without digestive tract malformations (control) were collected. Immunohistochemical staining was employed to examine the expression of c-kit and Cx43 (immunomarkers of ICCs) in gastric tissues. RESULTS: The muscular layer of the stomach wall became thinner or deficient in the gastric perforation group. C-kit and Cx43 positive cells in gastric tissues decreased significantly in the gastric perforation group compared with those in the control group (P＜0.01). CONCLUSIONS: The development of spontaneous neonatal gastric perforation is associated with the decreased quantity of ICCs and damaged gap junction structure of the stomach wall.