变态反应性疾病儿童血浆肥大细胞羧肽酶和类糜蛋白酶含量的检测

Measurement of plasma mast cell carboxypeptidase and chymase levels in children with allergic diseases

摘要:

目的：测定变态反应性疾病患儿血浆中肥大细胞羧肽酶和类糜蛋白酶的含量，评价其在变态反应性疾病诊断中的意义。方法：采用ELISA法检测59例变态反应性疾病儿童和53例健康儿童血浆中肥大细胞羧肽酶和类糜蛋白酶水平。结果：变态反应性疾病患儿血浆羧肽酶和类糜蛋白酶含量分别为1.089±0.752 ng/mL、0.905(0.375~2.318)ng/mL，显著高于健康儿童［0.593±0.380 ng/mL、0.454(0.097~1.077) ng/mL］，差异有统计学意义（P<0.05)。变态反应性疾病患儿血浆羧肽酶水平与类糜蛋白酶水平呈显著正相关(r=0.684，P<0.01)。结论：变态反应性疾病儿童血浆中肥大细胞羧肽酶和类糜蛋白酶水平增高，提示二者可作为变态反应性疾病的诊断的有意义的指标。

Abstract:

OBJECTIVE: To evaluate the roles of plasma mast cell carboxypeptidase and chymase in the diagnosis of allergic diseases by measuring the contents of both in children. METHODS: A total of 59 children with allergic diseases and 53 healthy children were recruited into the study. Plasma levels of mast cell carboxypeptidase and chymase were measured using ELISA. RESULTS: The plasma levels of mast cell carboxypeptidase and chymase in children with allergic children were 1.089±0.752 ng/mL and 0.905(0.375-2.318) ng/mL, respectively, which were significantly higher than those in healthy children ［0.593±0.380 ng/mL and 0.454 (0.097-1.077) ng/mL respectively; P<0.05］.There was a significantly positive correlation between plasma mast cell carboxypeptidase and chymase levels in children with allergic diseases (r=0.684, P<0.01). CONCLUSIONS: Plasma levels of mast cell carboxypeptidase and chymase increase in children with allergic diseases, suggesting that mast cell carboxypeptidase and chymase may serve as the indexes for the diagnosis of allergic diseases.