宫内窘迫大鼠脑组织促红细胞生成素及其受体的表达

Expression of erythropoietin and its receptor in the brain of newborn rats suffering from fetal distress

摘要:

目的：观察促红细胞生成素(erythropoietin，EPO)及其受体(EPOR)在宫内窘迫大鼠生后脑组织的动态表达规律,并探讨其意义。方法：采用结扎足月妊娠待产时母鼠双侧子宫动脉10 min 后行剖宫产制作新生大鼠宫内窘迫的模型作为实验组,研究新生大鼠生后不同时间点（0 h、2 h、6 h、12 h、24 h、48 h、3 d和7 d）脑组织EPO及EPOR的动态表达情况,并同时检测血清EPO的水平。未结扎双侧子宫动脉的剖宫产新生大鼠作为对照组。结果：与对照组相比，实验组EPO蛋白和mRNA的表达在2 h、6 h、12 h等3个时间点明显增强（均P<0.05）；EPOR蛋白和mRNA的表达在2 h、6 h、12 h、24 h、48 h、3 d等6个时间点均明显增加（均P<0.05）。实验组血清EPO水平在生后2 h高于对照组,差异有统计学意义（t=2.52,P=0.02）。结论：宫内窘迫新生大鼠生后脑组织EPO和EPOR在短期内迅速增加，而且EPOR高表达持续时间较EPO长。这一发现为外源性EPO治疗胎儿宫内窘迫致新生儿脑损伤提供了有力依据。

Abstract:

OBJECTIVE: To study the expression of erythropoietin (EPO) and its receptor (EPOR) in the brain of newborn rats suffering fetal distress. METHODS: A model of fetal distress was prepared by ligating bilateral uterine arteries of the rats with full-term pregnancy for 10 minutes before cesarean sections. The expression levels of EPO and EPOR in the brain of newborn rats were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot at 0, 2, 6, 12, 24, 48, 72 hrs and 7 days after birth. Serum EPO levels were measured using ELISA simultaneously. The newborn rats born by cesarean sections which were not subjected to uterine artery ligation were used as the control group. RESULTS: The expression of EPO protein and mRNA in brain tissues in the fetal distress group increased significantly compared with the control group 2, 6 and 12 hrs after birth (P<0.05). The expression of EPOR protein and mRNA in brain tissues in the fetal distress group increased significantly compared with the control group 2, 6, 12, 24 and 48 hrs, and 3 days after birth (P<0.05). Serum EPO levels in the fetal distress group were significantly higher than in the control group 2 hrs after birth. CONCLUSIONS: The EPO and EPOR levels in the brain increase quickly after birth in newborn rats suffering from fetal distress. The EPOR is high expressed for a longer time than EPO. This can provide a basis for the treatment of neonatal brain damage induced by fetal distress by exogenous EPO.