环孢素对慢性再生障碍性贫血患儿外周血T调节细胞及Foxp3表达的影响

Effect of cyclosporine on regulatory T cells and Foxp3 in the peripheral blood of children with chronic aplastic anemia

摘要:

目的：探讨再生障碍性贫血（简称再障）患儿应用环孢素治疗后外周血中CD4+CD25+CD127low T调节细胞（Treg）及Foxp3 mRNA表达水平的变化。方法：选取50例慢性再障（CAA）患儿，随机分为环孢素治疗组（30例）及非环孢素治疗组（20例），同时选取健康儿童20例作为对照组。采用流式细胞术检测各组患儿CD4+CD25+CD127low Treg的表达，并用实时荧光定量PCR检测Foxp3 mRNA的水平。结果：治疗6个月后，环孢素治疗组外周血CD4+CD25+CD127low Treg和Foxp3 mRNA表达水平与对照组比较差异无统计学意义；而非环孢素治疗组两者表达水平显著低于对照组，差异有统计学意义；环孢素治疗组两者表达水平明显高于非环孢素治疗组，差异有统计学意义。结论：环孢素可以促进再障患儿外周血CD4+CD25+CD127low Treg及Foxp3 mRNA表达水平上升。

Abstract:

OBJECTIVE: To explore the expression diversification of CD4+CD25+CD127low regulatory T (Treg) cells and Foxp3 mRNA in the peripheral blood of children with aplastic anemia after the treatment with cyclosporine. METHODS: Fifty children with chronic aplastic anemia were enrolled, among whom 30 received cyclosporine treatment (cyclosporine group) and 20 were treated with conventional methods (conventional group). Twenty healthy children were enrolled as the control group. The expression of CD4+CD25+CD127low Treg cells was detected by flow cytometry. The expression of Foxp3 mRNA was detected by real-time Q-PCR. RESULTS: The expressions of Foxp3 mRNA and CD4+CD25+CD127lowTreg cells showed no significant difference between the cyclosporine and the control groups 6 months after treatment. On the contrary, there were significantly lower expressions of both in the conventional group than in the control group (P<0.05). Meanwhile, the cyclosporine group had significantly higher expressions of Foxp3 mRNA and CD4+CD25+CD127low Treg cells than the conventional group (P<0.05). CONCLUSIONS: The expressions of CD4+CD25+CD127low Treg cells and Foxp3 mRNA in children with aplastic anemia increase after cyclosporine treatment.