儿童急性白血病Notch1和Jagged1的基因表达

Gene expression of Notch1 and Jagged1 in children with acute leukemia

摘要:

目的：探讨儿童急性白血病（AL）骨髓或外周血单个核细胞中Notch1和Jagged1基因表达及其在AL发病中的可能作用。方法：收集2009年2月至2011年7月初诊的47例AL患儿和20例正常或非恶性血液病儿童（对照组）骨髓或外周血单个核细胞，采用二步法半定量逆转录聚合酶链反应（RT-PCR）方法检测骨髓或外周血中Notch1和Jagged1基因表达情况。47例AL患儿中，急性淋巴细胞白血病（ALL）32例，其中B-ALL 26例，T-ALL 6例；急性髓细胞白血病（AML）15例。结果：ALL组及AML组Notch1基因阳性率高于对照组，差异均有统计学意义（P<0.05）。T-ALL患儿Notch1 基因表达水平高于B-ALL患儿，差异有统计学意义（P<0.01）。ALL组及AML组Jagged1基因阳性率与对照组比较差异无统计学意义，但ALL组及AML组Jagged1表达水平均高于对照组，差异有统计学意义（P<0.05）。结论：Notch1在儿童不同类型ALL中的表达有明显差异，T-ALL 患儿中的Notch1表达明显高于B-ALL患儿；在儿童AL细胞中，普遍存在Notch1信号的活化；Notch1在AML儿童中异常表达，提示Notch1在AML儿童中也起着重要作用。Jagged1在ALL及AML儿童中异常表达，但需要收集更多资料论证。

Abstract:

OBJECTIVE: To study the gene expression of Notch1 and Jagged1 in children with acute leukemia (AL) and their possible roles in the pathogenesis of AL. METHODS: Mononuclear cells from bone marrow or peripheral blood of 47 children with AL and 20 controls (normal children or children with nonmalignant hematologic disease) were collected from February 2009 to July 2011. A two-step method to semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the gene expression of Notch1 and Jagged1. Of the 47 children with AL, there were 26 cases of B-ALL, 6 cases of T-ALL and 15 cases of AML. RESULTS: The positive expression rate of Notch1 in the ALL and AML groups was higher than in the control group (P<0.05). The expression level of Notch1 in T-ALL children was higher than in B-ALL children (P<0.01). The positive expression rate of Jagged1 in the ALL and AML groups was not significantly different from the control group, however, the expression level of Jagged1 in the ALL and AML groups was higher than in the control group (P<0.05). CONCLUSIONS: There are significant differences in the gene expression of Notch1 between children with different types of ALL, and a higher expression of Notch1 relates to T-ALL. The activation of Notch1 signal is common in children with AL. The abnormal gene expression of Notch1 in children with AML shows the role of Notch1 in AML. The gene expression of Jagged1 in children with ALL or AML is abnormal, and this needs to be confirmed by further research.