细菌脂多糖对哮喘小鼠血清IL-4、IL-8及肺组织VEGF表达的影响

Effects of bacterial lipopolysaccharide on serum IL-4, serum IL-8 and pulmonary VEGF expression in mice with asthma

摘要:

目的：观察细菌脂多糖（LPS）对支气管哮喘小鼠血清IL-4、IL-8及肺组织血管内皮生长因子（VEGF）的影响，探讨LPS在哮喘发生过程中的调节作用。方法：27只BALB/c小鼠随机分为对照组、哮喘组和LPS干预组，每组9只。采用ELISA法检测哮喘小鼠血清IL-4、IL-8的浓度，免疫组织化学染色法检测肺组织中VEGF的表达。结果：哮喘组血清IL-4和IL-8水平明显高于对照组（P<0.05）；而LPS干预组IL-4和IL-8水平明显低于哮喘组（P<0.05），但仍高于对照组（P<0.05）。哮喘组气道VEGF表达显著高于对照组（P<0.05）；LPS干预组其表达明显低于哮喘组（P<0.05），但仍高于对照组（P<0.05）。结论：LPS可能通过降低哮喘小鼠血清IL-4和IL-8水平及气道VEGF表达，减轻气道炎症，减少气道血管重塑的发生。

Abstract:

OBJECTIVE: To study the regulatory role of bacterial lipopolysaccharide (LPS ) in the development of bronchial asthma by examining the effects of LPS on serum IL-4, serum IL-8 and pulmonary vascular endothelial growth factor (VEGF) expression in mice with asthma. METHODS: Twenty-seven BALB/c mice were randomly assigned into control, asthma and LPS-treated asthma groups (n=9 each). Serum IL-4 and IL-8 concentrations were measured using ELISA. VEGF expression in lung tissues was examined using the immunohistochemical method. RESULTS: Serum IL-4 and IL-8 concentrations in the asthma group were significantly higher than in the control group (P<0.05). LPS treatment significantly decreased serum IL-4 and IL-8 concentrations compared with the asthma group (P<0.05), although levels were significantly higher than in the control group (P<0.05). Airway VEGF expression in the asthma group was significantly higher than in the control group (P<0.05). LPS treatment significantly decreased airway VEGF expression compared with the asthma group (P<0.05), although concentrations remained higher than in the control group (P<0.05). CONCLUSIONS: LPS can decrease serum IL-4, serum IL-8 and pulmonary VEGF expression in mice with asthma, and thus can possibly reduce both airway inflammation and airway vascular remodeling.