穿心莲内酯对哮喘小鼠嗜酸性粒细胞表达的影响及其作用机制

Effects of andrographolide on the expression of eosinophil granulocytes and possible mechanisms

摘要:

目的：穿心莲内酯是中药穿心莲的主要有效成分，在体内外均具有较强的抗炎作用。该研究观察穿心莲内酯对哮喘小鼠支气管肺泡灌洗液（BALF）中嗜酸性粒细胞、eotaxin及IL-5表达的影响。方法：32只BALB/c小鼠随机分为对照组、哮喘组、布地奈德组、穿心莲内酯组，每组8只。以卵清蛋白（OVA）致敏激发建立哮喘小鼠模型。ELISA法检测BALF及外周血中eotaxin、IL-5的水平，实时荧光定量PCR法检测肺组织eotaxin、IL-5 mRNA的表达。结果：穿心莲内酯明显抑制哮喘小鼠BALF中嗜酸性粒细胞（P0.05）。结论：穿心莲内酯通过降低哮喘小鼠eotaxin、IL-5的表达，抑制哮喘气道嗜酸性粒细胞浸润。

Abstract:

OBJECTIVE: Andrographolide, the active component in andrographis paniculata, has potent anti-inflammatory actions. This study aimed to evaluate the effects of andrographolide on eosinophil granulocytes (EOS) and the expression of eotaxin and IL-5 in mice with asthma. METHODS: BALB/c mice were randomly assigned into normal control, asthma, budesonide treatment and andrographolide treatment groups (n=8 each). Mice in the latter three groups were sensitized and challenged with ovalbumin (OVA) to induce asthma. ELISA was used to detect the concentrations of eotaxin and IL-5 in bronchoalveolar lavage fluid (BALF) and peripheral blood. The expression of eotaxin mRNA and IL-5 mRNA in lung tissues was detected by real-time quantitative PCR. RESULTS: Andrographolide treatment significantly decreased EOS count in BALF (P<0.05) and the effect of andrographolide was better than the effect of budesonide. Andrographolide treatment significantly down-regulated the expression of eotaxin and IL-5 in BALF, lung eotaxin mRNA expression and blood IL-5 expression (P<0.05), but the effects of andrographolide were poorer than the effects of budesonide. Andrographolide treatment resulted in a decrease in blood eotaxin expression and lung IL-5 mRNA expression and the effects of andrographolide were similar to budesonide. CONCLUSIONS: Andrographolide can down-regulate the expression of IL-5 and eotaxin and thus suppress the inflitration of EOS in a mouse model of asthma.