机械通气前后早产儿动脉血中IFN-γ和IP-10的变化

Changes to IFN-γ and IP-10 in the arterial blood of premature infants before and after mechanical ventilation

摘要:

目的：观察机械通气前后早产儿动脉血中炎症因子IFN-γ和趋化因子IP-10的变化，探讨机械通气引起肺损伤可能的免疫炎症机制。方法：纳入符合标准的患儿23例，在机械通气前和通气后4 h取动脉血检测，用ELISA法测定炎症因子IFN-γ和趋化因子IP-10的变化。结果：患儿机械通气后4 h动脉血中IFN-γ和IP-10分别为105±54 pg/mL、220±80 pg/mL，较机械通气前（分别59±40 pg/mL、130±67 pg/mL）均明显升高（P<0.01）。结论：机械通气后血清IFN-γ和IP-10水平增高，提示IFN-γ和IP-10可能参与了机械通气所致肺损伤的免疫炎症过程。

Abstract:

OBJECTIVE: This study examined changes to pro-inflammatory cytokine IFN-γ and chemokine IP-10 in the arterial blood of premature infants before and after mechanical ventilation, with the aim of exploring possible mechanisms of ventilation-induced lung injury. METHODS: Twenty-three neonates requiring mechanical ventilation were enrolled in this study. Arterial blood samples were collected for measuring IFN-γ and IP-10 levels using ELISA before and 4 hours after mechanical ventilation. RESULTS: Blood IFN-γ and IP-10 increased significantly from 59±40 pg/mL and 130±67 pg/mL respectively before mechanical ventilation to 105±54 pg/mL and 220±80 pg/mL respectively after 4 hours of mechanical ventilation (P<0.01). CONCLUSIONS: Serum IFN-γ and IP-10 levels increase after mechanical ventilation, suggesting that both may participate in the immune-inflammatory process of ventilation-induced lung injury.