毛细支气管炎患儿血清25-（OH）D3与免疫球蛋白水平的变化

Serum levels of 25-(OH)D3 and immunoglobulin in children with bronchiolitis

摘要:

目的：研究毛细支气管炎患儿血清25-（OH）D3与免疫球蛋白水平的变化及临床意义。方法采用酶联免疫法检测35例毛细支气管炎患儿急性期和恢复期及20例健康儿童血清25-（OH）D3水平，速率散射比浊法测定免疫球蛋白含量。结果毛细支气管炎患儿急性期血清25-（OH）D3、IgG及IgA水平明显低于健康儿童组，而血清IgE明显高于健康儿童组，差异均有统计学意义（P＜0.05）。毛细支气管炎患儿恢复期血清25-（OH）D3水平较急性期增高，而IgE水平较急性期降低，差异有统计学意义（P＜0.05）。与健康儿童组比较，毛细支气管炎患儿恢复期血清25-（OH）D3及IgA水平明显降低，而血清IgE明显增高，差异亦有统计学意义（P＜0.05）。毛细支气管炎患儿急性期血清25-（OH）D3水平与IgG、IgA呈正相关（分别r=0.36，P＜0.05；r=0.63，P＜0.01），与IgE呈负相关（r=-0.72，P＜0.01）。毛细支气管炎患儿恢复期血清25-（OH）D3水平与IgE呈负相关（r=-0.34，P＜0.05）。结论毛细支气管炎患儿血清25-（OH）D3水平降低及免疫球蛋白水平失衡，提示25-（OH）D3及免疫球蛋白在其发病中起重要作用。

Abstract:

OBJECTIVE: To study the changes in the serum levels of 25-(OH)D3 and immunoglobulins in children with bronchiolitis, and the clinical significance of these changes. METHODS: Serum levels of 25-(OH)D3 were measured using ELISA in 35 children with bronchiolitis in the acute and recovery phases and 20 healthy children. Serum levels of immunoglobulins were determined by rate nephelometry. RESULTS: Compared with the healthy children, serum 25-(OH)D3, IgG and IgA levels in children with bronchiolitis in the acute phase were significantly lower and, in contrast, serum IgE levels were significantly higher (P＜0.05). Serum 25-(OH)D3 levels increased and serum IgE levels decreased significantly in the recovery phase compared with the acute phase in children with bronchiolitis (P＜0.05). However, compared with the healthy children, serum 25-(OH)D3 and IgA levels were significantly lower and serum IgE levels were significantly higher in children with bronchiolitis in the recovery phase (P＜0.05). Serum 25-(OH)D3 levels in children with bronchiolitis in the acute phase were positively correlated with serum IgG (r=0.36, P＜0.05) and IgA levels (r=0.63, P＜0.01), and negatively correlated with serum IgE levels (r=-0.72, P<0.01). A negative correlation was found between serum 25-(OH)D3 and IgE levels in children with bronchiolitis in the recovery phase (r=-0.34, P＜0.05). CONCLUSIONS: Serum 25-(OH)D3 levels decrease and there is immunoglobulin level imbalance in children with bronchiolitis, suggesting that 25-(OH)D3 and immunoglobulins may play important roles in the pathogenesis of bronchiolitis.