Gata4转录后蛋白共价修饰

Post-transcriptional protein modification of Gata4

摘要:

目的：Gata4是心脏发育重要的转录因子，其转录活性和DNA亲和力受转录后蛋白共价修饰的调节，并影响下游目的基因和相关转录因子表达，胚胎干细胞分化，心肌细胞生长和心脏发育。该文总结Gata4转录后蛋白共价修饰对其转录活性的影响，寻找其与心脏发育和先天性心脏病（congenital heart disease, CHD）的关系。结果发现乙酰化、磷酸化、SUMO化使其转录活性和DNA亲和力升高，下游基因表达上升，促进胚胎干细胞分化等呈正性调节；去乙酰化和甲基化下调Gata4转录活性，呈负性调节。因此，Gata4蛋白共价修饰对研究先天性心脏病及其他一些心脏疾病有重要的临床意义。

Abstract:

OBJECTIVE: Gata4 is an important transcription factor in heart development. Gata4 post-transcriptional protein modification regulates transcriptional activity and DNA binding, which in turn affects expression of downstream genes and transcription factors, differentiation of embryonic stem cells and cardiogenesis. This article summarizes the effect of post-transcriptional protein modification on transcriptional activity of Gata4 and the relationship between this effect and congenital heart disease. It was shown that acetylation, phosphorylation and SUMOylation upregulate transcriptional activity, DNA binding, downstream gene expression and embryonic stem cell differentiation. On the other hand, methylation and deacetylation downregulate Gata4 transcriptional activity. Post-transcriptional protein modification of Gata4 is very important in clinical research on congenital and other heart diseases.