缺氧缺血新生鼠脑内乙酰胆碱水平的变化

Acetylcholine level changes in neonatal rats with cerebral hypoxia and ischemia

摘要:

目的探讨缺氧缺血对新生鼠中枢胆碱能系统的远期影响及治疗措施。方法采用碱性羟胺比色法对缺氧缺血新生鼠（７日龄）损伤后即刻及其１４ｄ后左、右脑乙酰胆碱（Ａｃｈ）含量进行测定，并观察了损伤后即刻及３ｄ后腹腔内连续７ｄ注射４μｇ碱性成纤维生长因子（ｂＦＧＦ）对脑内Ａｃｈ含量的影响。结果缺氧缺血损伤后即刻，左脑Ａｃｈ含量显著降低，（Ｐ＜０．０５），于１４ｄ后仍未恢复；ｂＦＧＦ治疗对损伤后脑内Ａｃｈ含量无显著影响。结论缺氧缺血可损伤新生鼠中枢胆碱系统功能，此影响可持续至２１日龄。ｂＦＧＦ对胆碱能损伤无明显保护作用。

Abstract:

Objective To study the long-term effect of cerebral hypoxia and ischemia (HI) on the central acetylcholine (Ach) system and its treatments in neonatal rats. Methods Neonatal rats (7 days after birth) were subjected to permanent left common carotid artery ligation followed by 90 min of hypoxia with 10% oxygen at 36 ℃. The Ach level was measured immediately after HI (Group 1) and 14 days after HI (Group 2) using basic hydroxylamine chromatometry. In Group 3, basic fibroblast growth factor (bFGF) was injected (4μg ip) for 7 days immediately after HI. bFGF was given in the same way 3 days after HI in Group 4. The Ach level was measured 2 weeks after HI in Groups 3 and 4. Results The Ach level in the left globe decreased significantly immediately after HI (P<0.05). It did not return to normal until 14 days after HI. bFGF did not change the decreased Ach level obviously. Conclusions HI can damage the central Ach system of neonatal rat, moreover, the abnormality can persist for a long time. The bFGF has no protective effect on the damages of the Ach system .