黄芩甙、川芎嗪对兔感染性脑水肿与磷脂酶A_2的作用

Effect of baicalin and tetramethylpyrazine on infectious brain edema and phospholipase A2 in rabbits

摘要:

目的探讨黄芩甙（ＢＣ）、川芎嗪（ＴＭＰＺ）、甘露醇（ＭＮ）对兔百日咳菌液（ＰＢ）脑水肿的治疗作用及机理。方法应用兔感染性脑水肿模型，用光导纤维颅连续监测硬膜外颅内压，测定血清及脑脊液（ＣＳＦ）中磷脂酶Ａ２（ＰＬＡ２）活性、脑组织含水量（ＷＣ）、伊文思蓝（ＥＢ）、钠含量及丙二醛（ＭＤＡ）与超氧化物歧化酶（ＳＯＤ）活性，观察黄芩甙、川芎嗪和甘露醇治疗对血清及ＣＳＦ中ＰＬＡ２活性的抑制作用以及与改善脑水肿的关系。结果血清及ＣＳＦ中ＰＬＡ２活性ＢＣ组和ＴＭＰＺ组明显低于ＰＢ组和ＭＮ组。脑组织含水量、伊文思蓝含量与钠含量：ＰＢ组较ＮＳ组明显增高，治疗各组均减少，依次为ＭＮ组＞ＴＭＰＺ组＞ＢＣ组。３个治疗组均能降低ＭＤＡ浓度，提高ＳＯＤ活性和减轻颅内压增高。降颅内压的作用以ＢＣ最佳，依次为ＴＭＰＺ，ＭＮ。结论ＢＣ与ＴＭＰＺ均可抑制ＰＬＡ２活性和脂质过氧化，从而减轻脑水肿与降低颅内高压。

Abstract:

Objective To explore the mechanism and effect of baicalin (BC), tetramethy1pyrazine (TMPZ) and mannitol (MN) on infectious brain edema induced by pertussis bacilli (PB) in rabbits. Methods Epidural intracranial pressure (ICP) was monitored continuously by fiberoptic ICP monitor. The activity of phospholipase A 2 (PLA 2) in serum and CSF, brain water contents (WC), Evan′s blue (EB), Na + contents, and the concentrations of malondialdehy (MDA) and superoxide dismutase (SOD) were determined. Results The activity of PLA 2 in serum and CSF of both BC and TMPZ groups were significantly lower at 3 hours and 5 hours than those of PB and MN groups (P<0.05). WC, EB, Na + contents and MDA concentrations in left brain tissues of all treated groups were significantly lower compared with that of the PB group, but SOD activity in brain tissues of all treated groups were significantly higher than that of the PB group. All groups BC, TMPZ and MN could reduced the intracranial hypertension (ICH), the effect of BC was the strongest, that of TMPZ and MN in proper order. Conclusions Both BC and TMPZ could inhibit the activity of PLA 2 in serum and CSF, and reduce the brain edema and intracranial hypertension induced by PB in rabbits.