中枢神经系统疾病患儿血清和脑脊液髓鞘碱性蛋白的定量分析

Quantitative analysis of myelin basic protein in serum and cerebrospinal fluid in children with central nervous system diseases

摘要:

目的 探讨小儿中枢神经系统（ＣＮＳ） 疾病脑脊液（ＣＳＦ） 和血清髓鞘碱性蛋白（ ＭＢＰ） 水平的改变及临床意义，寻找反映小儿ＣＮＳ损伤的生化指标。方法 证实为ＣＮＳ疾病的患儿８５ 例，其中男５８ 例，女２７ 例，年龄１ 月～１３ 岁。采用ＥＬＩＳＡ 法对病例血清及部分病例ＣＳＦ标本作ＭＢＰ定量测定。结果 疾病组中ＣＮＳ感染、颅内出血、及颅内占位性病变等病例中ＣＳＦ和血清ＭＢＰ均值都明显高于正常对照组。结论 任何原因导致ＣＮＳ髓鞘结构的破坏时，其ＭＢＰ在ＣＳＦ的水平升高，当血清ＭＢＰ含量也升高时，提示血脑屏障（ＢＢＢ） 破坏，通透性升高。结果显示对ＣＳＦ和血清ＭＢＰ的检测，可作为一项反映ＣＮＳ损伤的生化指标。

Abstract:

Objective To investigate the change of myelin basic protein (MBP) in serum and CSF in children with central nervous system (CNS) diseases and its clinic significance, and to find a biochemistry index for CNS damage.Methods The ELISA method was used to determine the level of MBP in the serum and CSF of 85 cases of CNS diseases (58 males and 27 females, 1m～13y in age), including CNS infection, convalescence of HIE, delayed Vitmine K deficiency-induced intracranial hemorrhage, febrile convulsion and CNS tumor.Results Both means of MBP in serum and CSF were apparently higher in the CNS diseases group those of the control group. Conclusions There are different degree increase of MBP in serum and CSF in different CNS diseases, indicating a demyelinating pathological course and injurg of blood-brain barrier. The level of MBP in serum and CSF may taken as a biochemical index of reflecting CNS injury.