咽拭子在新生儿先天性人巨细胞病毒感染筛查中的应用

Application of throat swab nested PCR in the diagnosis of congenital human cytome-galovirus infection in neonates

摘要:

目的 探讨咽拭子PCR检测在新生儿先天性人巨细胞病毒（HCMV）活动性感染筛查中的临床应用价值。方法 收集51例新生儿的咽拭子和脐血标本，均进行HCMV gB的巢式PCR检测，其中18例进行脐血pp65抗原检测。结果 巢式PCR法检测咽拭子HCMV gB基因的灵敏度和特异性分别为67%和75%，其阳性预测值和阴性预测值分别为57%和82%。结论 咽拭子HCMV gB的巢式PCR检测，具有无创、快速、特异的优点，有望成为新生儿HCMV先天性活动性感染筛查的最佳检测手段。

Abstract:

Objective To evaluate the clinical application value of throat swab nested PCR for detecting active congenital human cytomegalovirus (HCMV) infection in neonates. Methods The throat swabs and umbilical cord blood specimens from 51 neonates were collected for nested PCR assay for HCMV glycoprotein B (gB) gene. Moreover, 18 of them were subjected to a pp65 antigen test. Results The sensitivity and specificity of throat swab nested PCR for HCMV gB gene were 67% and 75%, respectively, and the positive and negative predictive values were 57% and 82%, respectively. Conclusions Throat swab nested PCR assay for HCMV gB gene is non-invasive, rapid, and highly sensitive for HCMV detection and holds promise as an excellent screening technology for detecting active congenital HCMV infection in neonates.