反复喘息婴幼儿外周血髓系抑制细胞检测及意义

Levels of myeloid-derived suppressor cells in peripheral blood of infants with recurrent wheezing

摘要:

目的 探讨外周血髓系抑制细胞（MDSCs）占单个核细胞比例在婴幼儿反复喘息发生、发展中的意义。方法 随机选取急性发作期的反复喘息婴幼儿31例作为喘息组，选取同年龄支气管肺炎患儿27例作为肺炎组；另选取同期在我院外科住院的患疝气、肾结石等非感染、非肿瘤性疾病术前患儿27例作为对照组。采用流式细胞术检测各组患儿外周血MDSCs占单个核细胞比例（MDSCs%）。结果 3组患儿外周血中MDSCs%差异有统计学意义（P<0.05），其中喘息组MDSCs%高于肺炎组和对照组（均P<0.05）。结论 反复喘息婴幼儿外周血中MDSCs高表达，可能在婴幼儿喘息反复发生、发展中起重要作用。

Abstract:

Objective To study myeloid-derived suppressor cells (MDSC) levels in peripheral blood of infants with recurrent wheezing, and the role of MDSC in the development of recurrent wheezing. Methods Thirty-one infants with recurrent wheezing at wheezing attacks were randomly enrolled in the study. Twenty-seven infants with bronchopneumonia and 27 preoperative infants (hernia or renal calculus), without infectious or neoplastic diseases, were selected as controls. The proportion of MDSC in peripheral blood mononuclear cells (PBMC) was measured by flow cytometry. Results The proportion of MDSC in PBMC in infants with wheezing was significantly higher than in those with bronchopneumonia and preoperative infants (P<0.05). Conclusions MDSC levels increase in infants with recurrent wheezing, suggesting that MDSC may play a crucial role in the development of this disorder.