MAOA-u VNTR多态性单独及和应激性生活事件交互作用与青少年重性抑郁障碍的关联分析

Association between MAOA-u VNTR polymorphism and its interaction with stressful life events and major depressive disorder in adolescents

摘要:

目的：探讨单胺氧化酶A基因相关多态区域（MAOA-u VNTR）基因多态性与青少年重性抑郁障碍（major depressive disorder, MDD）有无关联，以及其与应激性生活事件（stressful life events, SLEs）之间的交互作用与MDD之间有无关联。方法：394名研究对象（MDD患者187人，正常对照207人）采用SNaP-shot系统进行基因分型，评估受试者近1年内的SLEs，采用统计学软件比较各配对组别MAOA-u VNTR基因型及等位基因的分布；建立基因×环境（GXE）交互作用的二分类logistic回归模型，分析MAOA-u VNTR基因型与SLEs交互作用与青少年MDD患病的关联性。结果：MAOA-u VNTR 基因型及等位基因分布与青少年MDD是否发生、抑郁严重程度、是否共病焦虑、是否出现自杀观念/行为/企图均无直接相关性；男性及女性MAOA-u VNTR基因型与SLEs均不存在和青少年MDD患病相关的交互作用。结论：尚不能认为MAOA-u VNTR与青少年MDD相关；MAOA-u VNTR与SLEs间不存在与青少年MDD相关联的基因-环境交互作用。

Abstract:

OBJECTIVE: To investigate whether the genetic polymorphism, upstream variable number of tandem repeats (uVNTR), in the monoamine oxidase A (MAOA) gene, is associated with major depressive disorder (MDD) in adolescents and to test whether there is gene-environment interaction between MAOA-uVNTR polymorphism and stressful life events (SLEs). METHODS: A total of 394 Chinese Han subjects, including 187 adolescent patients with MDD and 207 normal students as a control group, were included in the study. Genotyping was performed by SNaP-shot assay. SLEs in the previous 12 months were evaluated. The groups were compared in terms of the frequency distributions of MAOA-uVNTR genotypes and alleles using statistical software. The binary logistic regression model of gene-environment interaction was established to analyze the association of the gene-environment interaction between MAOA-u VNTR genotypes and SLEs with adolescent MDD. RESULTS: The distribution profiles of MAOA-u VNTR genotypes and alleles were not related to the onset of MDD, severity of depression, comorbid anxiety and suicidal ideation/behavior/attempt in adolescents. The gene-environment interaction between MAOA-u VNTR genotypes and SLEs was not associated with MDD in male or female adolescents. CONCLUSIONS: It is not proven that MAOA-u VNTR polymorphism is associated with adolescent MDD. There is also no gene-environment interaction between MAOA-u VNTR polymorphism and SLEs that is associated with adolescent MDD.