再生障碍性贫血患儿骨髓造血干细胞端粒酶活性及相关基因表达的研究

Expression of telomerase activity and its related genes in the marrow hemopoietic stem cells of children with aplastic anemia

摘要:

目的：探讨再生障碍性贫血（简称再障）患儿骨髓造血干细胞端粒酶活性及端粒酶逆转录酶（hTERT）基因和端粒酶RNA组分（hTERC）基因的表达变化和关系。方法：用重复序列扩增（TRAP）银染法和实时荧光定量RT-PCR法分别检测52例慢性再障患儿（CAA组）、13例急性再障患儿（SAA组）和21例骨髓造血正常儿童（对照组）端粒酶活性及hTERT和hTERC mRNA的表达。结果：CAA组、SAA组端粒酶活性和hTERT mRNA水平均高于对照组（P<0.01），且CAA组端粒酶活性和hTERT mRNA水平较SAA组升高（P<0.05）；hTERC mRNA在各组间表达差异无统计学意义（P=0.812）；hTERTmRNA的表达水平与端粒酶活性呈正相关（r＝0.660，P＜0.01）。结论：端粒酶活性的表达变化与儿童再障发病及发展过程有关，hTERT表达在端粒酶活性调节过程中起重要作用。

Abstract:

OBJECTIVE: To explore the relationship of telomerase RNA component (hTERC) and the telomerase reverse transcriptase (hTERT) with telomerase activity in the marrow hemopoietic stem cells of children with aplastic anemia (AA). METHODS: Fifty-two children with chronic AA, 13 children with acute AA and 21 normal controls were enrolled in the study. Telomerase activity and the expression of mRNA of hTERT and hTERC were detected by Telomeric Repeat Amplification Protocol (TRAP) with silver staining and real-time Q-PCR respectively. RESULTS: Levels of telomerase activity in both the chronic and acute AA groups were higher than in the control group (P<0.01). The AA groups had significantly higher expression of hTERT mRNA than the control group (P<0.01). The chronic AA group had higher expression of hTERT mRNA and telomerase activity than the acute AA group (P<0.05). There was no significant difference in the expression of hTERC mRNA among the three groups (P=0.812). There was a significant correlation between the expression of hTERT mRNA and telomerase activity (r=0.660, P<0.01). CONCLUSIONS: Expression of telomerase activity may be involved in the pathophysiology and development of AA, and hTERT plays a crucial role in expression of telomerase activity.