CD4+CD25high+CD127low调节性T 细胞在毛细支气管炎患儿外周血中的表达改变及其临床意义

Changes to CD4+CD25high+CD127low regulatory T cells in peripheral blood from children with bronchiolitis, and its clinical significance

摘要:

目的：探讨CD4⁺CD25^high+CD127^low调节性T 细胞（regulatory T cell, Treg）在毛细支气管炎患儿外周血中的数量变化及其临床意义。方法：随机选取31例2岁以下毛细支气管炎患儿作为毛细支气管炎组，同期住院的同年龄段支气管肺炎患儿25例和患有疝气、肾结石等非感染性疾病患儿25例分别作为支气管肺炎组和对照组，采用流式细胞术检测3组患儿外周血中CD4⁺CD25^high+CD127^lowTreg 占CD4+T 细胞百分比。结果：毛细支气管炎组患儿外周血中CD4⁺CD25^high+CD127^lowTreg 占总CD4+T 细胞的8.0%±2.1%，低于支气管肺炎组（9.6%±2.6%）及对照组（11.3%±2.9%），差异有统计学意义（P<0.05）。结论：毛细支气管炎患儿外周血CD4⁺CD25^high+CD127^lowTreg水平可能是一个较好的反映毛细支气管炎患儿免疫功能状态的参考指标，其在外周血中数量下降提示Treg细胞可能参与毛细支气管炎的发生发展。

Abstract:

OBJECTIVE: To study changes to CD4⁺CD25^high+CD127^low regulatory T cells (Treg) in peripheral blood from children with bronchiolitis, and to explore its clinical significance. METHODS: Thirty-one children with bronchiolitis and aged under two years were randomly enrolled as the bronchiolitis group, and 25 under two-year-olds with bronchopneumonia were randomly enrolled as the bronchopneumonia group. A further twenty-five children with non-infectious diseases such as hernia and renal calculus served as the control group. The level of CD4⁺CD25^high+CD127^lowTreg in peripheral blood was measured by multi-color detection and multi-parameter flow cytometry. RESULTS: The proportion of CD4⁺CD25^high+CD127^lowTreg in peripheral blood in the bronchiolitis group (8.0%±2.1%) was significantly lower than in the bronchopneumonia (9.6%±2.6%; P<0.05) and control groups (11.3%±2.9%; P<0.05). CONCLUSIONS: CD4+CD25high+CD127lowTreg level in peripheral blood may be an index of immunological function in infants. A decreased level of CD4⁺CD25^high+CD127^lowTreg in peripheral blood suggests that Treg cells may be involved in the pathogenesis and development of bronchiolitis.