1,25-(OH)2D3对哮喘小鼠肺内TIM-4表达的影响

Effect of 1,25-(OH)2D3 on expression of TIM-4 in the lungs of asthmatic mice

摘要:

目的：建立小鼠哮喘气道重塑模型，观察1,25-(OH)2D3对哮喘小鼠气道结构及T细胞免疫球蛋白域粘蛋白域蛋白-4（T cell immunoglobulin mucin protein-4, TIM-4）表达的影响。方法：30只BALB/c雌性小鼠随机分为对照组、哮喘组和1,25-(OH)2D3干预组，采用卵清蛋白致敏、激发建立哮喘模型。取小鼠肺组织，采用苏木精-伊红染色观察气道重塑情况，并应用RT-PCR法和免疫组化法检测肺内TIM-4 mRNA和蛋白的表达。结果：哮喘组发生典型的气道重塑改变，与对照组比较，TIM-4表达水平升高（105±9 vs 42±5，P<0.05）；1,25-(OH)2D3干预组较哮喘组气道重塑有所改善，TIM-4表达降低（78±6），差异有统计学意义（P<0.05）。结论：TIM-4可能参与小鼠气道重塑过程；新型免疫调节剂1,25-(OH)2D3可下调哮喘小鼠肺内TIM4的表达，改善气道重塑。

Abstract:

OBJECTIVE: To establish a mouse model of asthmatic airway remodeling and investigate the effects of 1,25-(OH)2D3 on airway structure and T cell immunoglobulin mucin protein-4 (TIM-4) expression in asthmatic mice. METHODS: Thirty female mice (BALB/c strain) were randomly divided into control, asthma and 1,25-(OH)2D3 intervention groups. An asthmatic mouse model was induced using ovalbumin. Lung tissue of the mice was collected, mRNA expression of TIM-4 was evaluated by RT-PCR and airway remodeling and protein expression of TIM-4 were observed by hematoxylineosin staining and immunohistochemistry.RESULTS: Typical airway remodeling was found in the asthma group, and TIM-4 expression in this group was significantly higher than in the control group (105±9 vs 42±5; P<0.05). Compared with the asthma group, the 1,25-(OH)2D3 intervention group showed improvement in airway remodeling and a decrease in TIM-4 expression (78±6) (P<0.05). CONCLUSIONS: TIM-4 may be involved in the airway remodeling of mice. As a new type of immunoregulator, 1,25-(OH)2D3 can downregulate expression of TIM-4 in the lungs and improve airway remodeling in asthmatic mice.