新生大鼠高氧肺损伤P38活化和MMP-2 mRNA表达的研究

Expression of P38 MAPK and MMP-2 mRNA in neonatal rats with hyperoxia-induced lung injury

摘要:

目的：观察高氧肺损伤新生大鼠肺组织中P38-丝裂原活化蛋白激酶（P38）的活化与基质金属蛋白酶-2（MMP-2）mRNA表达水平的变化，并探讨P38的活化对MMP-2 mRNA表达的影响。方法：将36只新生Sprague-Dawley大鼠随机分为空气组、高氧组和SB203580干预组，每组12只。建立高氧肺损伤动物模型，处理组给予相应的干预。各组分别于第3天和第7天处死大鼠，常规苏木精-伊红染色，观察肺组织病理学变化；Western blot检测肺组织P38蛋白的活化水平，RT-PCR检测肺组织MMP-2 mRNA表达的变化。结果：高氧组大鼠肺组织P38的活化和MMP-2 mRNA表达水平较空气组和干预组明显增强，差异有统计学意义（P<0.01）。结论：P38在新生大鼠高氧肺损伤中活化增强，可能参与MMP-2 mRNA表达的调控。

Abstract:

OBJECTIVE: To observe P38 mitogen-activated protein kinase (P38 MAPK) and matrix metalloproteinase-2 (MMP-2) mRNA expression level changes in neonatal rats with hyperoxia-induced lung injury,and to investigate the influence of P38 MAPK activation on MMP-2 mRNA expression. METHODS: Thirty-six Sprague-Dawley (SD) rats were randomly divided into three groups: air control, hyperoxia and SB203580-treated hyperoxia (n=12). The rats were sacrificed on the 3rd and 7th days and the lungs were removed. Hematoxylin-eosine staining was used to observe the pathological changes in lung tissues. RESULTS: Compared with the air and SB203580-treated groups, levels of P38 MAPK and MMP-2 mRNA significantly increased in the hyperoxia group (P<0.01). CONCLUSIONS: Expression of P38 MAPK increases in neonatal rats with hyperoxia-induced acute lung injury and this may play a role in control of the expression of MMP-2 mRNA.