回族及维吾尔族肥胖儿童瘦素基因多态性分析

Leptin gene C2549A polymorphism in minority Hui and Uygur children with obesity

摘要:

目的 探讨瘦素基因型与回族及维吾尔族儿童肥胖之间的关系。方法 从乌鲁木齐周边地区6所小学选取68例肥胖儿童（回族35例，维吾尔族33例）及69例非肥胖儿童（回族36例，维吾尔族33例），共137例儿童纳入该研究。空腹12 h后抽取2组儿童外周静脉血5 mL，常规提取基因组DNA，应用限制性片段长度多态性方法检测其C2549A瘦素基因型，同时检测各组儿童空腹血脂水平，采用放射免疫法测定其空腹血瘦素和胰岛素水平。结果 137例受试儿童中，瘦素基因C2549A AA、AC、CC基因型频率分别为9.5%、33.6%、56.9%。A等位基因频率在回族及维吾尔族儿童中的差异有统计学意义（P<0.05）；A等位基因频率、AA+AC基因型频率在回族及维吾尔族肥胖组和非肥胖组间比较差异均无统计学意义（P >0.05）。含有AA+AC、CC基因型的回族和维吾尔族儿童肥胖组及非肥胖组间血瘦素水平比较差异无统计学意义。结论 回族与维吾尔族儿童存在瘦素基因C2549A多态性；单个瘦素基因多态性与回族及维吾尔族儿童肥胖的发病无明显相关性。

Abstract:

Objective To investigate the relationship of leptin gene polymorphism with obesity in ethnic minority Hui and Uygur children in China. Methods Sixty-eight ethnic minority (35 Hui and 33 Uygur) children with obesity and 69 age-matched minority (36 Hui and 33 Uygur) children without obesity were recruited from six primary schools in the sub-urban areas of Urumqi. Venous blood was sampled from all subjects after fasting for 12 hours. Leptin gene C2549A polymorphism was determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism methods. Blood concentrations of lipids, leptin and insulin were measured with biochemical methods and radioimmunoassys, respectively. Results In the 137 children tested, the prevalence of AA, AC and CC genotype was 9.5%, 33.6% and 56.9%, respectively. A allele frequency was significantly different between the two ethnic (i.e. Hui and Uygur) groups (P<0.05). A allele frequency and AA+ AC genotype frequency were not significantly different between obese and non-obese children in both ethnic groups (P >0.05). Blood leptin levels were not significantly different between obese and non-obese children with an AA+AC or CC genotype in both ethnic groups (P >0.05). Conclusions Leptin gene polymorphisms exist in Hui and Uygur children. The C2549A polymorphism is not significantly associated with the prevalence of obesity in both Hui and Uygur children.