髓系相关蛋白8和14在川崎病患儿中的表达及其与循环内皮细胞的关系

Expression of myeloid-related protein complex in association with circulating endothelial cells in children with acute Kawasaki disease

摘要:

目的 研究髓系相关蛋白8、14（MRP-8、-14）在川崎病（KD）患儿中的表达及其与循环内皮细胞数（CECs）的关系。方法 选取41 例KD患儿为研究对象，根据有无冠状动脉扩张分为冠状动脉无扩张KD组和冠状动脉扩张KD组，同时选取年龄和性别与KD患儿相匹配的因急性上呼吸道感染发热的40例患儿为对照组。分别通过ELISA、RT-PCR和流式细胞术检测各组患儿外周血MRP-8/MRP-14二聚体含量、单个核细胞MRP-8和MRP-14 mRNA表达水平及CECs。Pearson积差相关分析法分析MRP-8/MRP-14二聚体含量与CECs的相关性。结果 冠状动脉扩张KD组患儿MRP-8/MRP-14二聚体含量、单个核细胞MRP-8和MRP-14 mRNA表达水平及CECs均高于冠状动脉无扩张KD组（均P<0.05），且两组上述指标均高于对照组（均P<0.05）；MRP-8/MRP-14二聚体含量与CECs呈正相关（r=0.69，P<0.05）。结论 MRP-8、MRP-14表达增高在KD的发病中起重要作用，且与CECs增高显著相关，是引起KD患儿血管损伤、冠状动脉扩张的重要因素。

Abstract:

Objective To investigate the expression of myeloid-related protein complex (MRP-8/14) in children with acute Kawasaki Disease (KD). Methods A total of 41 children with acute KD and 40 age-and sex-matched control children with upper respiratory tract infection were recruited. Serum levels of MRP-8/MRP-14 complex were measured by ELISA, messenger ribonucleic acid (mRNA) abundance of MRP-8 and MRP-14 in circulating granulocytes and monocytes was determined by RT-PCR, and the number of circulating endothelial cells was determined by flow cytometry. Results When the analysis was stratified according to the presence or absence of coronary artery ectasia in the KD patient group, serum levels of MRP-8/MRP-14 complex, MRP-8 and MRP-14 mRNA abundance in granulocytes, and the number of circulating endothelial cells were all significantly higher in KD patients with coronary artery ectasia than in KD patients without coronary artery ectasia (P<0.05). Serum levels of MRP-8/MRP-14 complex were positively correlated with the number of endothelial cells in the circulation (r=0.69, P<0.05). Conclusions Serum levels of MRP-8/MRP-14 complex are elevated in a positive association with the number of circulating endothelial cells in KD children with coronary artery ectasia, suggesting a causative role in the development of coronary artery lesions.