0~6岁儿童淋巴细胞免疫分型影响因素探讨

Influencing factors for lymphocyte subsets in children aged 0-6 years

摘要:

目的 探讨影响0~6 岁儿童淋巴细胞免疫分型的因素.方法 采集45 例健康足月儿脐动脉血及79 例健康儿童静脉血,按不同生产方式将45 例足月儿分为顺产组(n=22)和剖宫产组(n=23);按不同年龄段将健康儿童分为28 d~ 组(n=25)、1 岁~ 组(n=26)和3~6 岁组(n=28),用流式细胞术检测淋巴细胞亚群值,分析生产方式、性别和年龄对淋巴细胞免疫分型的影响.结果 顺产和剖宫产两组新生儿在T 和Ts 细胞计数、B 细胞百分比、NK 细胞百分比和绝对计数等指标上差异均有统计学意义(均P<0.05);各淋巴细胞免疫表型绝对计数和百分比在不同性别健康儿童之间比较差异均无统计学意义(均P>0.05);除Ts 和NK 细胞计数外,其余各亚群在不同年龄组儿童之间差异均有统计学意义(均P<0.05).结论 儿童淋巴细胞亚群的分布受年龄因素的影响较大,新生儿出生时也受生产方式的影响;在建立儿童淋巴细胞免疫分型正常参考范围时应按年龄分段.

Abstract:

Objective To investigate the influencing factors for lymphocyte subsets in children 0 to 6 years of age.Methods Umbilical artery blood samples from 45 healthy full-term infants and venous blood samples from 79 healthychildren between 0 and 6 years were collected. According to the methods of delivery, the full-term infants were dividedinto vaginal delivery group (n=22) and cesarean section group (n=23). Healthy children were divided into different agegroups:28 days to 12 months (n=25), 1-3 years (n=26), and 3-6 years (n=28). Lymphocyte subsets were examined byflow cytometry. The influencing factors including delivery method, sex, and age, which might have an effect on the lymphocyte subsets, were analyzed. Results There were significant differences in T and Ts cell counts, percentage of Bcells, and percentage and count of natural killer (NK) cells between the full-term infants of vaginal delivery and cesareansection (P<0.05). The absolute counts and percentages of different lymphocyte subsets showed no significant differences between males and females in healthy children (P>0.05). The counts of all lymphocyte subsets except Ts and NK cellsvaried significantly between different age groups (P<0.05). Conclusions Lymphocyte subsets in children under 6years of age are more profoundly affected by age. Delivery method is also a contributing factor in newborn infants. Thereference range of lymphocyte subsets in children should be established for different age groups.