动态监测婴儿肠道16SrDNA宏基因组5例报告

Dynamic changes of intestinal 16S rDNA metagenome in 5 infants

摘要:

目的 探讨婴儿肠道16S rDNA 宏基因组的动态变化.方法 收集5 例健康婴儿生后3 d、1 个月、6 个月、1 岁时粪便样本共17 份,提取细菌总DNA,采用新一代高通量测序技术对16S rDNA 的V6 高变区测序并进行物种分类、丰度及多样性分析.结果 17 份样品共产生原始测序数据为2 190.66 Mbp,Operational Taxonomic Units(OTU)数量36~308;优势菌门包括变形菌门、厚壁菌门、拟杆菌门和放线菌门;不同婴儿间肠道菌群分布有差异;科水平>1% 的物种生后3 d 时2~4 种,1 个月、6 个月时增至最多7 种,1 岁时达10 种;4 个时间点的npShannon 和Simpson 指数均值分别为1.117、1.460、2.088、2.50 和0.443、0.408、0.229、0.143.结论 婴儿粪便中含丰富细菌基因组;细菌物种丰度及分类存在个体差异;细菌多样性随日龄而增加,婴儿肠道菌群结构出生后逐渐趋向复杂和多样.

Abstract:

Objective To investigate the dynamic changes of intestinal 16S rDNA metagenome in healthy infants.Methods Seventeen fecal samples were collected at ages of 3 days, 1 month, 6 months and 1 year in 5 infants. Totalbacterial DNAs were extracted and submitted high throughout sequencing on the V6 viable region of 16S rDNA. Tagsand Operational Taxonomic Units (OTU) were then obtained and analysis of taxonomy, abundance and alpha diversitywere performed. Results In total 2 190.66 Mbp raw data in 17 samples were produced. The OTU numbers rangedfrom 36 to 308. The dominate phylum included Proteobacteria, Firmicutes and Bacteroidetes and Actinobacteria. The bacterial families >1% increased from only 2-4 per sample on day 3 to 7 at 1 or 6 months, 10 at 12 months. The averagenp Shannon and Simpson index on day 3, at 1 month, 6 months and 1 year were 1.117, 1.460, 2.088, 2.50 and 0.443, 0.408,0.229, 0.143 respectively. Conclusions Infants' intestines harbor abounding bacterial genomes. Distinct individual differences exist in infants in terms of intestinal bacterial abundance and composition. The abundance and diversity ofgut bacteria increase over time.