CD4+CD25+Foxp3+调节性T细胞与IL-33在儿童哮喘发病机制中的作用

Roles of CD4+CD25+Foxp3+ regulatory T cells and IL-33 in the pathogenesis of asthma in children

摘要:

目的 探讨CD4⁺CD25⁺Foxp3⁺调节性T细胞(Treg)与IL-33在儿童哮喘发病中的作用.方法 采用流式细胞仪检测45例哮喘患儿(哮喘组)、50例呼吸道合胞病毒感染喘息患儿(喘息组)及40例健康儿童(对照组)外周血CD4⁺CD25⁺Foxp3⁺Treg细胞百分比,采用ELISA法检测各组外周血血清IFN-γ、IL-4、IL-5及IL-33浓度,进行比较分析.结果 哮喘组患儿体内CD4⁺CD25⁺Foxp3⁺Treg 水平较喘息组及对照组均降低(P<0.05);哮喘组患儿体内IL-33水平较喘息组及对照组均升高(P<0.05),哮喘组患儿体内CD4⁺CD25⁺Foxp3⁺Treg与IL-33呈负相关(r=-0.156,P<0.01).结论 在哮喘患儿发病机制中,CD4⁺CD25⁺Foxp3⁺Treg与IL-33可能存在相互作用.

Abstract:

Objective To study the roles of CD4⁺CD25⁺Foxp3⁺ regulatory T cells (Treg) and IL-33 in the pathogenesis of asthma in children. Methods Flow cytometry was used to detect peripheral blood CD4⁺CD25⁺Foxp3⁺Treg proportion in CD4⁺T lymphocytes in.45 children with asthma, 50 children with wheezing caused by respiratory syncytial virus infection and 40 healthy children. Serum levels of IFN-γ, IL-4, IL-5 and IL-33 were measured using ELISA. Results The level of peripheral blood CD4⁺CD25⁺Foxp3⁺Treg in the asthma group was significantly lower than in the wheezing and control groups (P<0.05). In contrast, serum levels of IL-33 in the asthma group was significantly higher than in the wheezing and control groups (P<0.05). Peripheral blood CD4⁺CD25⁺Foxp3⁺Treg level was negatively correlated with serum IL-33 level in the asthma group(r=-0.156, P<0.01). Conclusions CD4⁺CD25⁺Foxp3⁺Treg may interact with IL-33 in the pathogenesis of childhood asthma.