甲磺司特对哮喘大鼠气道炎症及IL-5的影响

Effects of suplatast tosilate on airway inflammation and interleukin-5 in asthmatic rats

摘要:

目的 探讨甲磺司特（suplatast tosilate, IPD） 对哮喘大鼠IL-5 水平及气道炎症的影响。方法 50 只雄性成年Sprague-Dawley 大鼠（4 周龄）随机分为5 组：对照组、模型组、布地奈德组、IPD 早期干预组和IPD 晚期干预组，每组10 只。采用卵清白蛋白（OVA）致敏、激发，建立支气管哮喘大鼠气道炎症模型。观察肺泡灌洗液（BALF）炎性细胞总数和EOS 百分比，RT-PCR 检测肺组织IL-5 mRNA 的表达，ELISA测定BALF 上清液中IL-5 的含量。结果 模型组BALF 中炎性细胞总数及EOS 百分比、IL-5 含量及肺组织IL-5mRNA 表达量均较对照组明显增高（P< 0.05），IPD 干预组和BUD 组各指标均较模型组下降（P<0.05）。结论 IPD 可减轻哮喘气道炎症反应，可能与其抑制 IL-5 mRNA 的转录有关。

Abstract:

Objective To study the effects of suplatast tosilate (IPD) on the airway inflammation and expression of interleukin-5 in asthmatic rats. Methods Fifty adult male Sprague-Dawley rats (4-week-old) were randomly assigned to five groups: placebo control, untreated asthma, budesonide(BUD)-treated asthma, early or late IPD intervention group (n=10 rats each). Asthmatic mode was prepared by ovalbumin sensitizion and challenge. Inflammatory cells and the percentage of EOS were detected in bronchoalveolar lavage fluid (BALF). The lung tissues were removed to detect the lung histomorphology. Gene expression of IL-5 was measured by reverse transcription-polymerase chain reaction (RTPCR). Levels of interleukin 5 (IL-5) in BALF were measured using ELISA. Results The inflammatory cells and the percentage of EOS in BALF, IL-5 levels in BALF and IL-5 mRNA expression in the lung tissues were obviously higher in the untreated asthma group than the control group (P<0.05), while the parameters in the IPD or BUD-treated asthma groups were significantly lower than the untreated asthma group (P<0.05). Conclusions IPD treatment can alleviate airway inflammation in asthmatic rats, possibly through inhibiting IL-5 mRNA transcripts.