TGF-β1 和PAI-1 在早产儿支气管肺发育不良中的表达及意义

Expression of TGF-β1 and PAI-1 in premature infants with bronchopulmonary dysplasia

摘要:

目的 探讨支气管肺发育不良（BPD）与转化生长因子-1（TGF-β1）及纤溶酶原激活物抑制剂-1 （PAI-1）的关系。方法 回顾性分析2010 年1 月至2012 年12 月间胎龄≤ 32 周并存活28 d 以上极低出生体重儿96 例的临床资料，其中BPD 组21 例，非BPD 组75 例。采用ELISA 法测定2 组患儿血液中TGF-β1、PAI-1 的表达。结果 BPD 组TGF-β1 和PAI-1 在生后第7、14、21 天表达逐渐增高，且高于同时间点非BPD 组，差异有统计学意义（P<0.01），而非BPD 组在生后第7、14、21 天TGF-β1 及PAI-1 蛋白表达差异无统计学意义（P>0.05）。结论 BPD 组患儿血液中TGF-β1 及 PAI-1 表达增高，BPD 的发病过程可能与TGF-β1 及 PAI-1 高表达有关。

Abstract:

Objective To study the expression of transforming growth factor-β1 (TGF-β1) and plasminogen activator inhibitor-1 (PAI-1) and its significance in premature infants with bronchopulmonary dysplasia (BPD). Methods A retrospective analysis was performed on the clinical data of 96 very low birth weight infants (gestational age of ≤32 weeks) who survived for more than 28 days and were admitted to the Neonatal Intensive Care Unit between January 2010 and December 2012. These subjects were divided into BPD group (n=21) and non-BPD group (n=75). The expression of TGF-β1 and PAI-1 in blood was measured by ELISA. Results The levels of TGF-β1 and PAI- 1 in the BPD group increased gradually from the 7th day to the 14th day and then to the 21st day after birth, and were significantly higher than in the non-BPD group at all time points (P<0.01). The TGF-β1 and PAI-1 levels in the non-BPD group on the 7th, 14th, and 21st days after birth were not significantly different from each other (P>0.05). Conclusions The expression of TGF-β1 and PAI-1 in blood is elevated in premature infants with BPD, which may be associated with the development of BPD.