Toll样受体7和8在EV71感染死亡病例脑及肺组织中的表达及意义

Expression and significance of toll-like receptors 7 and 8 in brain and lung tissues of death cases caused by EV71 infection

摘要:

目的 通过检测肠道病毒71 型(EV71)重症感染死亡病例脑、肺组织中Toll 样受体(TLR)7、8 的表达,探讨TLR7、8 的表达在EV71 感染发病中的意义。方法 以9 例因EV71 感染死亡的儿童病例为研究对象(EV71 组),7 例因意外或非感染性疾病死亡的儿童病例作为对照。采集两组死亡病例尸检时的脑、肺组织,应用免疫组化法检测两组肺和脑组织中TLR7、8 的阳性表达;采用积分光密度值(IOD)对TLR7、8 的表达进行半定量分析。结果 免疫组化结果示EV71 组TLR7、8 在肺和脑组织中的表达呈强阳性,其IOD 值明显高于对照组(P<0.05)。TLR7 和TLR8 在EV71 组的肺、脑组织中的表达差异无统计学意义(P>0.05)。结论 TLR7、8 在EV71 重症感染死亡病例的脑、肺组织中呈高表达状态,提示TLR7、8 可能参与了EV71 重症感染患者脑及肺部损害的发病机制。

Abstract:

Objective To study the significance of toll-like receptors (TLR)-7 and-8 in the pathogenesis of infection caused by Enterovirus type 71 (EV71) through measuring the expression of TLR7 and TLR8 in brain and lung tissues from the death cases caused by EV71 infection. Methods Nine children who died of EV71 infection (EV71 group) were selected as study subjects, and 7 children who died of accidents or non-infectious diseases were used as the control group. Brain and lung tissues from the death cases in both groups at autopsy were collected, and immunohistochemistry was applied to detect the expression of TLR7 and TLR8 in lung and brain tissues in both groups. Integrated optical density (IOD) was applied for semi-quantitative analysis of the expression of TLR7 and TLR8. Results Immunohistochemical results showed that the expression of TLR7 and TLR8 in lung and brain tissues was strongly positive in the EV71 group, and the IOD values in the EV71 group were also significantly higher than those in the control group (P<0.05). There was no significant difference in the expression of TLR7 and TLR8 between lung and brain tissues in the EV71 group (P>0.05). Conclusions TLR7 and TLR8 are highly expressed in lung and brain tissues from the patients who die of severe EV71 infection, suggesting that TLR7 and TLR8 may be involved in the pathogenesis of brain and lung damages caused by severe EV71 infection.