TIAM1基因多态性与川崎病及其临床特点的相关性

Association of TIAM1 gene polymorphisms with Kawasaki disease and its clinical characteristics

摘要:

目的 探讨TIAM1基因多态性与川崎病(KD)及其临床特点的相关性。方法 采取病例-对照研究方法,选取2012年3月至2014年9月诊断为KD的患儿188例为KD组,同期选取197例行健康体检的儿童作为对照组。利用PCR-RFLP方法测定TIAM1基因2个SNP位点rs2833188和rs2833195的多态性分布,并进行统计分析。结果 KD组SNP位点(rs2833188)的基因型(AA、AG、GG)和等位基因分布频率与正常对照组相比差异均无统计学意义(P>0.05);KD组SNP位点(rs2833195)基因型(CC、GC、GG)分布频率与对照组相比差异有统计学意义(P=0.017),且KD患儿C等位基因频率高于对照组(P=0.015)。SNP位点(rs2833188)的多态性和KD患儿结膜充血的易感性相关(P=0.011);而SNP位点(rs2833195)的多态性与KD患儿皮疹的易感性相关(P=0.021)。结论 TIAM1基因的SNP位点rs2833195的多态性与KD的易感性相关;SNP位点rs2833188和rs2833195的多态性可能与KD患儿的部分临床特点的发生相关。

Abstract:

Objective To investigate the association of single nucleotide polymorphisms (SNP) rs22833188 and rs2833195 in TIAM1 gene with the susceptibility to Kawasaki disease (KD) and its clinical characteristic in children. Methods A case-control study was performed in this study. One hundred and eighty-eight children with KD and 197 normal children served as controls were enrolled. The genotypes of two SNPs rs22833188 and rs2833195 in TIAM1 gene were detected using PCR-RFLP. Results There were no significant differences in the genotype (AA, AG and GG) and allele frequencies of SNP rs2833188 between the KD and control groups. Significant differences in the genotype (CC, GC and GG) frequency of SNP rs2833195 were noted between the KD and control groups (P=0.017). The frequency of C allele in the KD group was higher than in the control group (P=0.015). The polymorphism of SNP rs2833188 was associated with the occurrence of rash (P=0.011), and the polymorphism of SNP rs2833195 was associated with the occurrence of conjunctival hyperemia (P=0.021). Conclusions The polymorphism of rs2833195 in TIAM1 gene is associated with the susceptibility to KD. The polymorphisms rs2833188 and rs2833195 in TIAM1 gene may be associated with some clinical characteristics in children with KD.