毛细支气管炎患儿外周血淋巴细胞CXCR3及IP-10的表达及意义

Roles of CXCR3 on lymphocytes and IP-10 of peripheral blood in infants with bronchiolitis

摘要:

目的 探讨趋化因子受体3(CXCR3)及γ干扰素诱导蛋白-10(IP-10)在毛细支气管炎(简称毛支)患儿外周血中的表达及临床意义.方法 随机选取毛支住院患儿55例,按有无过敏因素分为毛支Ⅰ组(有过敏因素)和毛支Ⅱ组(无过敏因素);同期住院的外科非感染患儿28例作为对照组.采用流式细胞术检测3组患儿外周血CD4⁺、CD8⁺淋巴细胞表面CXCR3(表面分子标记为CD183)的表达,ELISA 法测定血清中其配体IP-10的水平.结果 毛支Ⅰ组和毛支Ⅱ组外周血CD4⁺ T细胞表面CD183⁺细胞的表达及CD8⁺ T细胞表面CD183⁺细胞的表达均高于对照组(P<0.05),且毛支Ⅰ组高于毛支Ⅱ组(P<0.05);毛支Ⅰ组及毛支Ⅱ组患儿血清IP-10的浓度高于对照组(P<0.05),毛支Ⅰ组与毛支Ⅱ组比较差异无统计学意义.结论 CXCR3及IP-10参与了毛支的发病过程,且CXCR3与过敏因素有关.

Abstract:

Objective To study the roles of chemokine receptor 3 (CXCR3) on lymphocytes and interferon-γ-inducible protein-10 (IP-10) of peripheral blood in childhood bronchiolitis. Methods Fifty-five children with bronchiolitis were classified into Group I (with allergic factors) and Group II (without allergic factors). Twenty-eight children with noninfectious diseases were enrolled randomly as the control group. The expression of CXCR3 (CD183 as its molecular marker) on lymphocytes of peripheral blood was detected by flow cytometry. Serum IP-10 level was measured using ELISA. Results The expression of CD183⁺ cells on CD4⁺ and CD8⁺ lymphocytes in peripheral blood in children with bronchiolitis from both Group I and Group II was significantly higher than that in the control group (P<0.05), and Group I had higher expression of CD183⁺ cells on CD4⁺ and CD8⁺ lymphocytes than Group II (P<0.05).Serum IP-10 levels in Group I and Group II were significantly higher than those in the control group (P<0.05). However, there was no significant difference in serum IP-10 levels between Group I and Group II. Conclusions CXCR3 and IP-10 are involved in the pathogenesis of bronchiolitis, and CXCR3 is associated with allergic factors.