儿童传染性单核细胞增多症血浆蛋白质组学分析

Plasma proteomic analysis in children with infectious mononucleosis

摘要:

目的 通过对比分析传染性单核细胞增多症(infectious mononucleosis, IM)患儿与同龄健康儿童(对照组)的血浆蛋白质组图谱的差异表达,初步探索IM 特异表达或异常表达的血浆“分子标志物”以协助临床诊断。方法 采用双向电泳技术(2-DE)结合质谱技术对IM 患儿与对照组的血浆全蛋白进行比较蛋白质组学分析。结果 比较蛋白质组学分析发现IM 患儿组与对照组比较具有明显差异表达蛋白质共有7 个:血色素结合蛋白(Hpx)、VitD3 结合蛋白(DBP)、胎球蛋白A(fetuin A)、C- 反应蛋白(CRP)、载脂蛋白A(Apo A)、结合珠蛋白(HPT)、甲状腺运载蛋白(TTR),其中, HPT 表达量较对照组明显升高,其余蛋白的表达量均较对照组明显降低。结论 IM 患儿与对照组比较存在明显差异表达的蛋白质,这些差异蛋白表达量的改变均与肝脏的急性损伤有关,提示儿童IM 常伴有肝脏的急性损伤。若对这些差异表达的蛋白进一步分析,可望能够找到为IM 临床诊断和治疗提供一定参考价值的血浆特异性标志物。

Abstract:

Objective To explore the abnormal expression of plasma proteins by analysis of proteomic expression profile in children with infectious mononucleosis (IM). Methods Two dimensional gel electrophoresis (2-DE) followed by the mass spectrometry was used to examine important protein spots with different expression levels between children with IM and normal controls. Results Seven differential proteins were obtained: hemopexin, vitamin D binding protein, fetuin A, C-reactive protein, apolipoprotein A, haptoglobin and transthyretin. Compared with the control group, haptoglobin showed a higher expression level in children with IM, and the expression levels of the other proteins were obviously down-regulated. Conclusions The expression changes of differential proteins identified in this study are all related with the liver acute injury, suggesting that children with IM are associated with acute liver injury. Further studies on the characteristics of above proteins will contribute to the diagnosis and treatment of pediatric IM.