Foxp3及NFAT1在再生障碍性贫血患儿中的检测及意义

Measurement of Foxp3 and NFAT1 in children with aplastic anemia

摘要:

目的 研究再生障碍性贫血(简称再障)患儿治疗前后外周血中Foxp3 及NFAT1 蛋白表达水平的变化,探讨其在再障发病中的作用。方法 提取60 例正常儿童(对照组)和68 例治疗有效的再障患儿治疗前后外周血中的单个核细胞,采用免疫印迹法检测Foxp3、NFAT1 蛋白的表达,并分析Foxp3、NFAT1 蛋白表达的相关性及其与外周血血红蛋白(Hb)、白细胞(WBC)及血小板(PLT)数量的相关性。结果 再障组患儿治疗前(发病期)外周血中Foxp3、NFAT1 蛋白的表达较对照组明显降低(P<0.05);治疗后(恢复期)的表达较发病期明显升高(P<0.05)。Foxp3 与NFAT1 蛋白的表达呈正相关(r=0.812,P<0.05)。再障组患儿治疗后恢复期的Foxp3 和NFAT1 蛋白表达水平均与外周血Hb、WBC 及PLT 数量呈正相关(P<0.05)。结论 再障患儿外周血中Foxp3、NFAT1 蛋白表达水平降低,提示其在再障的发病过程中起重要作用;外周血Foxp3、NFAT1 蛋白表达水平在一定程度上可以提示病情的变化。

Abstract:

Objective To study the expression of Foxp3 and NFAT1 protein in peripheral blood (PB) in children with aplastic anemia (AA) and their roles in the pathogenesis of AA. Methods The expression levels of Foxp3 and NFAT1 protein of mononuclear cells in PB were measured by Western blot in 68 children with AA before and after treatment and in 60 normal children (control group). The correlation between Foxp3 and NFAT1 protein expression and the correlation of the Foxp3 and NFAT1 protein expression with blood Hb, WBC and platelet levels were analyzed. Results The expression levels of Foxp3 and NFAT1 protein in PB in the acute phase in the AA group were significantly lower than in the control group (P<0.05). After treatment (recovery phase) the expression levels of Foxp3 and NFAT1 protein increased obviously compared with those in the acute phase (P<0.05). The Foxp3 protein level was positively correlated with the NFAT1 protein level (r=0.812, P<0.05). Both the Foxp3 and NFAT1 protein levels were positively correlated with blood Hb, WBC and platelet levels in children with AA in the recovery phase (r=0.537, 0.579, 0.655 respectively; P<0.05). Conclusions The Foxp3 and NFAT1 protein levels in PB are reduced in children with AA, suggesting that they are involved in the pathogenesis of AA. The measurement of Foxp3 and NFAT1 protein levels may be useful in the severity evaluation of AA.