反复喘息的婴幼儿外周血髓源性抑制细胞和Th17细胞的检测及意义

Measurement of myeloid-derived suppressor cells and T-helper 17 cells in peripheral blood of young children with recurrent wheezing

摘要:

目的 探讨反复喘息婴幼儿外周血髓源性抑制细胞(MDSC)及Th17 细胞比例及临床意义。方法 随机选取急性发作期的反复喘息婴幼儿30 例作为喘息组,同时选取同年龄段20 例支气管肺炎患儿作为肺炎组,另选取同年龄段在该院外科住院的疝气、肾结石等非感染性、非肿瘤性疾病术前患儿23 例作为对照组。流式细胞术检测各组患儿外周血中MDSC、Th17 细胞的比例,采用Spearman 相关性分析MDSC 及Th17 细胞比例的相关性。结果 喘息组患儿MDSC 占有核细胞的百分比明显高于肺炎组和对照组,肺炎组高于对照组(P<0.05)。喘息组Th17 细胞占单个核细胞的百分比明显高于肺炎组及对照组,肺炎组与对照组Th17 比较差异无统计学意义。喘息组外周血MDSC 及Th17 细胞比例呈正相关关系(r=0.645,P<0.01)。结论 MDSC 及Th17 细胞可能参与了婴幼儿反复喘息的发生发展。

Abstract:

Objective To determine the frequencies and significance of myeloid-derived suppressor cells (MDSCs) and T-helper 17 (Th17) cells in peripheral blood of young children with recurrent wheezing. Methods Thirty young children with an acute exacerbation of recurrent wheezing were randomly enrolled. Twenty age-matched children with bronchopneumonia (pneumonia group) and 23 age-matched preoperative children with non-infectious or nonneoplastic diseases (hernia or renal calculus) (control group) were selected. The frequencies of MDSCs and Th17 cells in the peripheral blood were measured using flow cytometry and their correlation was determined by the Spearman’s correlation coefficient. Results The percentage of MDSCs in nucleated cells was significantly higher in the wheezing group than in the pneumonia and control groups (P<0.05), and it was significantly higher in the pneumonia group than in the control group (P<0.05). The percentage of Th17 cells in mononuclear cells was significantly higher in the wheezing group than in the pneumonia and control groups (P<0.05), but it showed no significant difference between the pneumonia and control groups (P>0.05). The frequency of MDSCs was positively correlated with the frequency of Th17 cells in the wheezing group (r=0.645, P<0.01). Conclusions MDSCs and Th17 cells may contribute to the pathogenesis of recurrent wheezing in young children.