RAD50基因多态性与儿童急性淋巴细胞白血病的相关性

Association between polymorphism of RAD50 gene and acute lymphoid leukemia in children

摘要:

目的 探讨RAD50基因的SNP位点(rs17166050)的多态性与我国中部地区儿童急性淋巴细胞白血病(ALL)的相关性。方法 177例来自湖北武汉或其周边地区的ALL患儿和232例健康儿童作为研究对象。177例患儿中, 标危66例, 中危69例, 高危42例。利用PCR-RFLP的方法检测RAD50基因SNP位点多态性, 研究该多态性与ALL易感性及临床危险度的相关性。结果 ALL组的RAD50基因SNP位点的基因型(AA、GA、GG)分布与对照组相比差异有统计学意义(P=0.038), 且G等位基因与ALL易感性显著相关(OR=1.459, 95%CI：1.034~2.057, P=0.031); 但在ALL组中, 该SNP位点的多态性与ALL的危险度不相关。结论 RAD50基因的SNP位点(rs 17166050)的多态性与儿童ALL的易感性相关, 但与其危险度分层不具有相关性。

Abstract:

Objective To investigate the association between single nucleotide polymorphism (SNP) (rs17166050) in RAD50 gene and acute lymphoid leukemia (ALL) in children. Methods A total of 177 ALL children from Wuhan and surrounding areas and 232 healthy children were selected. The numbers of standard-risk, medium-risk, and high-risk children were 66, 69, and 42, respectively. The genotypes of SNP in RAD50 gene were determined using PCR-RFLP, and the relationship of the RAD50 polymorphism with ALL susceptibility and clinical risk was analyzed. Results The genotype (AA, GA, and GG) distribution of SNP in RAD50 gene showed significant differences between the ALL and control groups (P=0.038), and G allele was significantly associated with ALL susceptibility (OR=1.459, 95% CI: 1.034-2.057, P=0.031). However, the SNP was not associated with the risk stratification of ALL. Conclusions The SNP (rs17166050) in RAD50 gene is associated with the susceptibility to ALL in children, but is not associated with the risk stratification of ALL.